利用Caco-2细胞模型模拟槲皮素和芦丁在小肠的吸收

doi:国家自然科学基金资助课题(30371757)

利用Caco-2细胞模型模拟槲皮素和芦丁在小肠的吸收

王海玲¹,刘宁²,刘志强²,刘忠英¹,张寒琦³ ,周秋丽¹

1.吉林大学药学院生化分析教研室,吉林长春 130021; 2.中科院长春应用化学研究所新药研究室,吉林长春 130022；3. 吉林大学化学学院分析化学教研室,吉林长春 130012)

收稿日期:2006-03-21 修回日期:1900-01-01 出版日期:2007-01-28 发布日期:2007-01-28
通讯作者: 刘忠英

Absorption of quercetin and rutin across human intestinal epithelial by using Caco-2 cells

WANG Hai-ling¹,LIU Ning²,LIU Zhi-qiang² ,LIUZhong-ying¹,ZHANG Han-qi³ ,ZHOU Qiu-li¹

1.Department of Biochemistry Analysis, School of Pharmacy, Jilin University, Changchun 130021,China;2.Laboratory of New Drug Research, Changchun Institute of Applied Chemistry,CAS, Changchun 130022,China; 3.Department of Chemistry Analysis, School of Chemistry, Jilin University,Changchun 130012,China

Received:2006-03-21 Revised:1900-01-01 Online:2007-01-28 Published:2007-01-28
Contact: LIUZhong-ying

摘要/Abstract

摘要： 目的：探讨药物分子槲皮素、芦丁的小肠吸收情况。方法：利用Caco-2细胞模型观察药物分子由细胞绒毛面（A面）到基底面（B面）、基底面（B面）到细胞绒毛面（A面）两个方向的转运过程。结果：由细胞绒毛面（A面）到基底面（B面）槲皮素的表观渗透系数（P_app）=(5.15±0.65)×10^-6cm•s^-1 ，芦丁P_app=(0.05±0.01)×10^-6cm•s^-1,由基底面（B面）到细胞绒毛面（A面）槲皮素及芦丁的P_app分别为(10.54±1.35)×10^-6cm•s^-1和(0.07±0.01)×10^-6cm•s^-1 。结论：槲皮素、芦丁可以通过小肠上皮细胞吸收进入体内，槲皮素较芦丁更易于吸收。

关键词: 槲皮素, 芦丁, 肠吸收, 表观渗透系数

Abstract: To study the absorption of quercetin and rutin in human intestinal epithelial. MethodsBy using Caco-2 cell line as an intestinal epithelial cell model, the transportatrions of quercetin and rutin were studied from apical side to basolateral side or from basolateral side to apical side. ResultsThe apparent permeabilities (Papp) of quercetin and rutin from the apical side to basolateral side were( 5.15±0.65)×10^-6cm•s^-1 and (0.05±0.01)×10^-6cm•s^-1, respectively. The Papp of quercetin and rutin from basolateral side to apical side were (10.54±1.35)×10^-6cm•s^-1 and ( 0.07±0.01)×10^-6cm•s^-1, respectively. Conclusion Quercetin and rutin can be absorbed across intestinal epithelial cells, moreover qercetin is easier to be absorbed than rutin.

Key words: quercetin , rutin, intestinal absorption, apparent permeability

中图分类号:

R329.25

王海玲,刘宁,刘志强,刘忠英,张寒琦 ,周秋丽. 利用Caco-2细胞模型模拟槲皮素和芦丁在小肠的吸收[J]. J4, 2007, 33(1): 33-36.

WANG Hai-ling,LIU Ning,LIU Zhi-qiang ,LIUZhong-ying,ZHANG Han-qi ,ZHOU Qiu-li. Absorption of quercetin and rutin across human intestinal epithelial by using Caco-2 cells[J]. J4, 2007, 33(1): 33-36.

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