激活素受体相互作用蛋白4的基因克隆

doi:国家自然科学基金资助课题（30400400; 3044

激活素受体相互作用蛋白4的基因克隆

王世瑶¹，柳忠辉¹，牟大鹏²，张红军¹，杨琼¹，常颖¹，台桂香¹

1.吉林大学基础医学院免疫学教研室，吉林长春 130021；2．吉林大学第二医院眼科，吉林长春 130041

收稿日期:2006-03-29 修回日期:1900-01-01 出版日期:2007-01-28 发布日期:2007-01-28
通讯作者: 台桂香

Cloning of novel activin receptor-interacting protein 4 gene

WANG Shi-yao¹, LIU Zhong-hui¹, MU Da-peng², ZHANG Hong-jun¹,YANG Qiong¹, CHANG Ying¹, TAI Gui-xiang¹

1. Department of Immunology, School of Basic Medical Sciences, Jilin University, Changchun 130021, China; 2. Department of Ophthalmology, Second Hospital, Jilin University, Changchun 130041, China)

Received:2006-03-29 Revised:1900-01-01 Online:2007-01-28 Published:2007-01-28
Contact: TAI Gui-xiang

摘要/Abstract

摘要： 目的：克隆新的与激活素受体ⅡA（ActRⅡA）相互作用蛋白，即激活素受体相互作用蛋白4 （ActRIP4）基因。方法：应用ActRIP2基因片段作为探针，从小鼠cDNA文库中钓取ActRIP4 cDNA；采用哺乳细胞双杂交进一步确定ActRIP4 与ActRⅡA的相互作用；RT-PCR检测ActRIP4 mRNA在组织中的表达情况。结果：克隆的ActRIP4基因全长691bp，ORF区编码 118个氨基酸残基；ActRIP4与ActRⅡA具有特异性结合作用；天然的ActRIP4 mRNA在小鼠多种组织中表达。结论：ActRIP4属于ActRIP家族新成员，可以与ActRⅡA相互作用，可能在多种组织中介导激活素受体信号传导过程。

关键词: 受体相互作用蛋白, 哺乳动物细胞双杂交, 逆转录聚合酶链反应

Abstract: To clone the gene of a novel activin receptor-interacting protein 4 (ActRIP4) which interacts with type Ⅱ activin receptor (ActRⅡA). Methods The ActRIP4 gene from a mouse cDNA library was cloned by using the ActRIP2 gene fragment as the probe. By mammalian cell two-hybrid, its ability to combine with ActRⅡA was confirmed. The expressions of ActRIP4 mRNA in tissues were analyzed by RT-PCR. Results The cloned full-length cDNA for ActRIP4 was 691bp, which ORF was encoding 118 amino acids. The specific mammalian cell two-hybrid analysis revealed that ActRIP4 could specifically combine with ActRⅡA. The results of RT-PCR showed that ActRIP4 mRNA expressed in many tissues. Conclusion ActRIP4 is a novel member of ActRIP family, and has the ability to specifically combine with ActRⅡA, which may mediate signaling transduction of activin receptor in various tissues.

Key words: receptor-interacting protein, mammalian cell two-hybrid, reverse transcriptase polymerase chain reaction

中图分类号:

R392.12

王世瑶，柳忠辉，牟大鹏，张红军，杨琼，常颖，台桂香. 激活素受体相互作用蛋白4的基因克隆[J]. J4, 2007, 33(1): 37-40.

WANG Shi-yao, LIU Zhong-hui, MU Da-peng, ZHANG Hong-jun,YANG Qiong, CHANG Ying, TAI Gui-xiang. Cloning of novel activin receptor-interacting protein 4 gene[J]. J4, 2007, 33(1): 37-40.

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