妊娠高血压综合征患者脐血肾上腺髓质素和内皮素的测定及其在脐带血管的表达

doi:吉林省科技厅资助课题

妊娠高血压综合征患者脐血肾上腺髓质素和内皮素的测定及其在脐带血管的表达

阮利红¹，王鑫²，潘宇³，朱凤全¹

1. 吉林大学第二医院妇产科，吉林长春130041；2.吉林省人民医院心血管内科，吉林长春130021；3. 长春市中心医院妇产科，吉林长春130051

收稿日期:2003-09-23 修回日期:1900-01-01 出版日期:2004-05-28 发布日期:2004-05-28

Deternination of adrenomedullin and endothelin in cord bloodand their expressions in umbilical cord vessel ofpatients with pregnancy-induced hypertension

RUAN Li-hong¹,PAN Yu²,ZHU Feng-quan¹

1. Department of Obstetrics and Gynecology, Second Hospital, Jilin University,Changchun 130041,China;2. Deparment of Angiocardiopathy,The People′s Hospital of Jilin Province,Changchun 130021,China；3. Deptartment of Obstetrics and Gynecology, Central Hospital of Changchun,Changchun 130051,China

Received:2003-09-23 Revised:1900-01-01 Online:2004-05-28 Published:2004-05-28

摘要/Abstract

摘要： 目的：探讨脐带血和脐带血管肾上腺髓质素（ADM）和内皮素1（ET-1）在妊娠高血压综合征（简称妊高征）中的作用。方法：应用放射免疫法测定30例妊高征患者和12例正常妊娠晚期孕妇（简称正常晚孕组）脐静脉血浆ADM和ET-1水平，用免疫组化法检测ADM和ET-1蛋白在40例妊高征患者和12例正常妊娠晚期孕妇脐带血管中的表达。结果：①妊高征患者脐静脉血浆ADM水平高于正常晚孕妇女，且随着病情加重逐渐增高，中、重度妊高征患者与正常晚孕组比较差异也具有显著性(P<0.05)。妊高征患者脐静脉血浆ET-1水平略有升高，与正常晚孕组比较，差异无显著性(P>0.05)；②正常晚孕组脐带血管内皮细胞和平滑肌细胞有ADM的表达，妊高征组随着病情加重，ADM表达增强；正常晚孕妇女ET-1在脐带血管的内皮细胞表达，妊高征组随着病情加重，ET-1表达减弱。结论：脐血和脐带血管ADM和ET-1表达改变可能与妊高征胎儿胎盘之间血液循环调控有关。

关键词: EPH, 脐血, 肽类, 内皮缩血管肽1

Abstract: Objective To investigate the role of adrenomedullin (ADM) and endothelin-1 (ET-1) in the pathogenesis of pregnancy-induced hypertension (PIH). Methods The plasma concentrations of ADM in human umbilical vein of PIH patients（n=30） and normal late trimester pregnancy women （n=12）were measured by radioimmunoassay. The expressions of ADM and ET-1 in umbilical cord vessel of PIH patients（n=40）and normal late trimester pregnancy women （n=12） were detected by immunohistochemistry (SABC) . Results ①The plasma concentration of ADM in human umbilical vein of PIH patients was significantly higher than that of normal late trimester pregnancy women (P<0.05). The plasma concentration of ET-1 in human umbilical vein of PIH patients was similar to that of normal late trimester pregnancy women （P>0.05）. ②The expression of ADM was found in endothelium and smooth muscle cell of umbilical cord vessel，and it increased with the serious degree of PIH. The expression of ET-1 was only found in endothelium of umbilical cord vessel, and it decreased with the serious degree of PIH. Conclusion The changes of ADM and ET-1 in umbilical cord plasma and vessel may related to regulation of fetoplacental circulation in PIH.

Key words: EPH, fetal blood, peptides, endothelin-1

中图分类号:

R714.24

阮利红，王鑫，潘宇，朱凤全. 妊娠高血压综合征患者脐血肾上腺髓质素和内皮素的测定及其在脐带血管的表达[J]. J4, 2004, 30(3): 452-454.

RUAN Li-hong,PAN Yu,ZHU Feng-quan. Deternination of adrenomedullin and endothelin in cord bloodand their expressions in umbilical cord vessel ofpatients with pregnancy-induced hypertension[J]. J4, 2004, 30(3): 452-454.

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