超高压对CB3V毒力及免疫原性的影响

doi:国家自然科学基金资助课题（30472135）

超高压对CB₃V毒力及免疫原性的影响

郑贤红¹，李凡^1*，张守勤²

1.吉林大学基础医学院病原生物学教研室，吉林长春130021； 2.吉林大学生物与农业工程学院农业机械教研室，吉林长春 130025

收稿日期:2005-03-15 修回日期:1900-01-01 出版日期:2006-01-28 发布日期:2006-01-28
通讯作者: 李凡

Effects of high hydrostatic pressure on virulenceand antigenicity of Coxsackie virus B₃

ZHENG Xian-hong¹,LI Fan^1*, ZHANG Shou-qin²

1.Department of Pathogeniobiology, School of Basic Medical Sciences, Jilin University，Changchun 130021,China； 2. Department of Agricultural Mechanics,School of Biology and Agricultural Engineering,Jilin University，Changchun 130025, China

Received:2005-03-15 Revised:1900-01-01 Online:2006-01-28 Published:2006-01-28
Contact: LI Fan

摘要/Abstract

摘要： 目的：通过超高压(HHP）对柯萨奇病毒B₃（CB₃V）毒力和病毒免疫原性的影响，探讨既能降低病毒毒力又能保留病毒免疫原性的有效方法，为制备CB₃V疫苗提供新的途径。方法：病毒毒力检测采用 50%组织细胞感染量试验（TCID₅₀）法；病毒免疫原性检测采用巨噬细胞吞噬功能测定、抗体检测、T淋巴细胞转化实验。结果：压力达到680 MPa时，病毒毒力降低，病毒TCID₅₀由10^-5上升为10^-4；高于700 MPa时，病毒被灭活。对病毒进行加热、压力减毒及压力灭活，小鼠巨噬细胞吞噬功能、抗体产生量及T淋巴细胞转化率与盐水对照组比较差异有显著性（P<0.05），加热组、减毒组与灭活组之间比较差异无显著性（P>0.05）。结论：CB₃V对压力的抵抗力强，灭活CB₃V的临界压力为700 MPa；压力灭活的CB₃V免疫原性不受影响。

关键词: 超高压, 巨噬细胞吞噬功能, 免疫原性

Abstract: Objective To evaluate the influences of high hydrostatic pressure (HHP) on the virulence and antigenicity of Coxsackie virus B₃ (CB₃V) , to explore a new physiological method to develop an active vaccine against Coxsackie virus. Methods The phagocytosis of the macrophages in mice to the CB₃V and the TCID₅₀ of the CB₃V before and after treated by HHP were examined. Results TCID₅₀ increased from 10^-5 to 10^-4 wh en treated by 680 MPa; viruses were inactivated at 700 MPa. Viruses were als o inactivated by the pressure less than 700 MPa, but needed prolonged time. Th e phagocytic function, antibody and T lymphocyte transformation rate in experime ntal mice (heated, attenuated, and inactive viruses) exhibited a significant dif ference compared with control mice (P<0.05). Conclusion CB₃V has a strong resistance to HHP. The inactivat ed pressure is about 700 MPa, however, the antigenicity of the virus is not infl uenced by the same value of HHP.

Key words: high hydrostatic pressure, phagocytic function of macrophage, antigenicity

中图分类号:

R372

郑贤红，李凡，张守勤. 超高压对CB₃V毒力及免疫原性的影响[J]. J4, 2006, 32(1): 8-3.

ZHENG Xian-hong,LI Fan, ZHANG Shou-qin. Effects of high hydrostatic pressure on virulenceand antigenicity of Coxsackie virus B₃[J]. J4, 2006, 32(1): 8-3.

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超高压对CB₃V毒力及免疫原性的影响

Effects of high hydrostatic pressure on virulenceand antigenicity of Coxsackie virus B₃

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