关键词: 毒性, 甲醛, 毒性, 联合染毒, 精子计数, 精子畸形率, 超氧化物歧化酶 tie-ji@126.con

Abstract: Objective To study the toxicity of benzene combining with formaldehyde on reproductive cells in mice. Methods Male mice were randomly divided into different groups:three experimental groups with different doses of benzene combining with formaldehyde (mg·kg^-1) (10+10,50+50 and 100+100), control group and vegetable oil group (n=10).　After 8 weeks by oral poison, the mice were killed，testis was weighed, sperm count was peformed, the rate of sperm deformation was counted and the SOD activity was measured. Results The quantities of sperm in various poison groups were obvious decreased and the rate of sperm deformation were obvious increased with dose increasing. Compared with control group, they both had obvious difference (P<0.05, P<0.01). Activities of SOD in testis in experimental groups we re obvious decreased with dose increasing. And when the doses of benzene combined with formaldehyde were (50+50) and (100+100)mg·kg^-1, there had obvious difference compared with control group (P<0.05, P<0.01). Conclusion Benzene combined with formaldehyde can cause obvious toxicity in reproductive cells.

Key words: toxicity, formaldehyde, toxicity, combing poison, sperm count, rate of sperm deformation , superoxide dismutase