甲基汞对豚鼠耳蜗外毛细胞外向钾电流的影响

doi:吉林省科技厅资助课题(990590)

甲基汞对豚鼠耳蜗外毛细胞外向钾电流的影响

刘淑芳¹，郭晓峰^1*，全成实²

1. 吉林大学第一医院耳鼻咽喉-头颈外科，吉林长春130021；2. 吉林大学基础医学院病理学教研室，吉林长春130021

收稿日期:2005-07-20 修回日期:1900-01-01 出版日期:2006-07-28 发布日期:2006-07-28
通讯作者: 郭晓峰

Effects of methylmercury on outward potassium currents in isolated outer hair cells of guinea pig cochlea

LIU Shu-fang¹, GUO Xiao-feng^1*,QUAN Cheng-shi²

1. Department of Otorhinolaryngology and Head-Neck Surgery, First Hospital，Jilin University,Changchun 130021,China；2. Department of Pathology,School of Basic Medical Sciences, Jilin University, Changchun 130021,China

Received:2005-07-20 Revised:1900-01-01 Online:2006-07-28 Published:2006-07-28
Contact: GUO Xiao-feng

摘要/Abstract

摘要： 目的：观察甲基汞对豚鼠耳蜗外毛细胞侧膜K+电流的影响，探讨甲基汞耳毒性的离子机制。方法：采用全细胞膜片钳技术分别观察汞污染度为1/1000 LD₅₀ 、1/100 LD ₅₀、1/10 LD₅₀对外毛细胞Ca²⁺敏感外向I_K（Ca）电流的影响。结果：汞中毒后I_K（Ca）幅值分别为（1.48±0.28）、（1.36±0.16）和（1.22±0.13）nA，与正常对照组相比分别下降了8%、16%和25%（P<0.05）。结论：甲基汞以浓度依赖方式抑制Ca2+敏感的外向K+电流，使I_K(Ca)幅值降低，最后导致听觉机能下降。这可能是甲基汞中毒的耳毒性机制之一。

关键词: 毛细胞, 外向钾电流, 膜片钳术

Abstract: Objective To investigate the effects of methylmercury on outward potassium currents in isolated outer hair cells of guinea pig cochlea. Methods The whole-cell patch clamp technique was used. Calcium sensitive outword potassium currents were recorded when the concentrations of mercurial were 1/1000 LD₅₀,1/100 LD₅₀ and 1/10 LD₅₀. Results After methylmercury poisoning, amplitudes of the outword potassium currents I_K(Ca) were (1.48±0.28)，(1.36±0.16) and （1.22±0.13）nA. Compared with control group, the amplitudes in three methylmercury poisoning groups were decreased by 8%,16% and 25%　(P<0.05). Conclusion Methylmercury can reduce I_K(Ca) in a dose-dependent manner, it may be one of the mechanisms of ototoxicity.

Key words: hair cells, outward potassium currents, patch clamp techniques

中图分类号:

R155.33

刘淑芳，郭晓峰，全成实. 甲基汞对豚鼠耳蜗外毛细胞外向钾电流的影响[J]. J4, 2006, 32(4): 612-614.

LIU Shu-fang, GUO Xiao-feng,QUAN Cheng-shi. Effects of methylmercury on outward potassium currents in isolated outer hair cells of guinea pig cochlea[J]. J4, 2006, 32(4): 612-614.

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