人β2糖蛋白1的重组表达及对抗磷脂抗体综合征患者血清诱导内皮细胞产生ICAM-1的影响

doi:教育部高等学校博士学科点专项基金资助课题

人β₂糖蛋白1的重组表达及对抗磷脂抗体综合征患者血清诱导内皮细胞产生ICAM-1的影响

付嘉，方艳秋，徐立，李明辉，刘桂英，姜艳芳，段秀梅，刘力华，许淑芬，谭岩^*

吉林大学第一医院中心实验室，吉林长春 130021

收稿日期:2006-01-19 修回日期:1900-01-01 出版日期:2006-07-28 发布日期:2006-07-28
通讯作者: 谭岩

Recombination of human β₂-glycoprotein-1 and its effect on expression of ICAM-1 in endothelial cell line induced by plasma from anti-phospholipid antibody syndrome patients

FU Jia, FANG Yan-qiu, XU Li, LI Ming-hui, LIU Gui-ying,JIANG Yan-fang,DUAN Xiu-mei,LIU Li-hua, XU Shu-fen,TAN Yan^*

Central Laboratary, First Hospital， Jilin University, Changchun 130021, China

Received:2006-01-19 Revised:1900-01-01 Online:2006-07-28 Published:2006-07-28
Contact: TAN Yan

摘要/Abstract

摘要： 目的：探讨重组人β₂糖蛋白1(hrβ₂-GP1)对抗磷脂抗体综合征(APS)患者血清诱导内皮细胞系产生细胞间黏附分子-1(ICAM-1)的影响。方法：应用pQE30表达载体表达hrβ₂-GP1，Western blotting对重组蛋白进行鉴定；应用hrβ₂-GP1处理前后的APS患者血清分别与内皮细胞株EA.hy926共孵育，细胞ELISA和 RT-PCR方法分析处理前后EA.hy926表达ICAM-1的变化。结果：成功构建pQE30-hβ₂-GP1，并对表达产物进行了纯化和鉴定，所获得的蛋白与预期的大小一致，并具有人β₂-GP1的抗原性；rhβ₂-GP1处理后的APS患者血清与处理前比较，与其孵育的内皮细胞系EA.hy926表达ICAM-1在蛋白和mRNA水平均明显降低，这种抑制作用随着rhβ₂-GP1的浓度增加而逐渐增强。结论：抗β₂糖蛋白1抗体(anti-β₂GP1)促进内皮细胞系EA.hy926表达ICAM-1, hrβ₂-GP1可中和此作用。

关键词: sub>糖蛋白1, 抗磷脂抗体综合征, 内皮细胞系, 细胞间黏附分子-1

Abstract: Objective To investigate the effect of human recombinant β₂-glycoprotein-1 (hrβ₂-GP1) on expression of intercellular adhesion molecular-1 (ICAM-1) in endothelial cell line induced by plasma from anti-phospholipid antibody syndrome (APS) patients. Methods hrβ₂-GP1 was expressed using pQE30 expression vector and characterized by Western blotting; hrβ₂-GP1 treated and untreated plasma from APS patients were incubated with endothilial cell line EA.hy 926. The changes of ICAM-1 expressed by EA.hy 926 were analyzed using cell-ELISA and RT-PCR. Results PQE30-hβ₂-GP1 was constructed, the exprssion products were purified and characterized. Compared with the untreated one, the membranous protein and mRNA expression of ICAM-1 in EA.hy926 of the hrβ₂-GP1 treated plasma from APS patients were significantly decreased. Conclusion Anti-β₂-GP1 antibody can elevate the expression of ICAM-1 in endothelial cell line EA.hy926， hrβ₂-GP1 can neutralize this effect.

Key words: sub>-glycoprotein-1, antiphospholipid antibody syndrome, endothelial cell line, intercellular adhesion molecular-1

中图分类号:

R392

付嘉，方艳秋，徐立，李明辉，刘桂英，姜艳芳，段秀梅，刘力华，许淑芬，谭岩. 人β₂糖蛋白1的重组表达及对抗磷脂抗体综合征患者血清诱导内皮细胞产生ICAM-1的影响[J]. J4, 2006, 32(4): 654-657.

FU Jia, FANG Yan-qiu, XU Li, LI Ming-hui, LIU Gui-ying,JIANG Yan-fang,DUAN Xiu-mei,LIU Li-hua, XU Shu-fen,TAN Yan. Recombination of human β₂-glycoprotein-1 and its effect on expression of ICAM-1 in endothelial cell line induced by plasma from anti-phospholipid antibody syndrome patients[J]. J4, 2006, 32(4): 654-657.

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人β₂糖蛋白1的重组表达及对抗磷脂抗体综合征患者血清诱导内皮细胞产生ICAM-1的影响

Recombination of human β₂-glycoprotein-1 and its effect on expression of ICAM-1 in endothelial cell line induced by plasma from anti-phospholipid antibody syndrome patients

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