激活素A对Neuro-2a细胞电压门控钠电流的作用

J4 ›› 2010, Vol. 36 ›› Issue (5): 837-840.

激活素A对Neuro-2a细胞电压门控钠电流的作用

孙洋¹, 柳忠辉¹, 王轶楠¹, 刘海岩², 王东辉¹, 葛敬岩³

1.吉林大学基础医学院免疫学教研室|吉林长春 130021；2.吉林大学基础医学院解剖学教研室|吉林长春 130021；3.吉林大学基础医学院生理学教研室|吉林长春130021

收稿日期:2010-04-15 出版日期:2010-09-28 发布日期:2010-09-28
通讯作者: 葛敬岩 (Tel:0431-85619472,E-mail:gejy@jlu.edu.cn) E-mail:gejy@jlu.edu.cn
作者简介:孙洋（1986-）|女,吉林省长春市人|在读医学硕士|主要从事神经免疫的研究。

Effects of activin A on voltage-gated Na+ current of Neuro-2a cells

SUN Xiang¹, LIU Zhong-Hui¹, WANG Yi-Nan¹, LIU Hai-Yan², WANG Dong-Hui¹, GE Jing-Yan³

1.Department of Immunology,School of Basic Medical Sciences,Jilin University,Changchun 130021,China;2.Department of Anatomy,School of Basic Medical Sciences,Jilin University,Changchun 130021,China;3.Department of Physiology,School of Basic Medical Sciences,Jilin University,Changchun 130021,China

Received:2010-04-15 Online:2010-09-28 Published:2010-09-28

摘要/Abstract

摘要：

目的：研究激活素A（activin A）对Neuro-2a细胞电压门控钠电流(INa）的作用及其机制，为激活素A在神经系统疾病中的应用奠定基础。方法：培养小鼠源性Neuro-2a细胞，分为IgG对照组和抗激活素受体ⅡA (ActRⅡA)抗体染色组，免疫组织化学染色观察ActRⅡA在Neuro-2a细胞中是否表达；将Neuro-2a细胞随机分为正常对照组和激活素A组，采用全细胞膜片钳技术检测激活素A对Neuro-2a细胞 INa 的作用；采用RT-PCR检测激活素A对血管活性肠肽(VIP)及诱导型一氧化氮合酶(iNOS) mRNA表达的影响。结果：正常Neuro-2a细胞中可检测到ActRⅡA的表达；与正常对照组比较，激活素A明显增加Neuro-2a细胞INa（P< 0.05）；激活素A组Neuro-2a细胞VIP mRNA表达明显高于正常对照组（P< 0.05），而iNOS mRNA表达低于正常对照组（P< 0.05）。结论：激活素A具有增加Neuro-2a细胞电压门控Na+电流的作用，VIP和iNOS途径可能参与了激活素A调控神经细胞的功能。

关键词: 激活素A；Neuro-2a细胞；电压门控性钠电流

Abstract:

Abstract:Objective To study the effect of activin A on voltage-gated sodium current(INa) of Neuro-2a cells and mechanism in order to lay a foundation for the application of activin A in nerve system diseases. Methods Neuro-2a cells were divided into IgG control group and anti-ActRⅡA antibody-stained group. Immunohistochemical staining was used to observe whether ActRⅡ A was expressed in Neuro-2a cells. The Neuro-2a cells were randomly divided into normal control group and activin A administration group. Whole cell patch clamp was used to analyze the effect of activin A on INa of Neuro-2a cells. The expressions of vasoactive intestinal peptide(VIP) and inducible nitric oxide synthase(iNOS) mRNA were examined by RT-PCR in Neuro-2a cells treated with activin A. Results ActRⅡA expression was detectable in normal Neuro-2a cells. Compared with control group,activin A increased INa in Neuro-2a cells significantly (P<0.05).The VIP mRNA expression in activin A group was higher than that in control group (P<0.05),while the expression of iNOS mRNA was lower than that in control group (P<0.05). Conclusion Activin A can increase INa of Neuro-2a cells and the effect of activin A on regulating the neuron function may be mediated via VIP and iNOS pathways.

Key words: activin A;neuro-2a cell;voltage-gated Na+ current

中图分类号:

R338.1

孙洋, 柳忠辉, 王轶楠, 刘海岩, 王东辉, 葛敬岩. 激活素A对Neuro-2a细胞电压门控钠电流的作用[J]. J4, 2010, 36(5): 837-840.

SUN Xiang, LIU Zhong-Hui, WANG Yi-Nan, LIU Hai-Yan, WANG Dong-Hui, GE Jing-Yan. Effects of activin A on voltage-gated Na+ current of Neuro-2a cells[J]. J4, 2010, 36(5): 837-840.