Skp2-P27kip1通路及其相关凋亡基因在结肠癌组织中的表达及意义

J4 ›› 2009, Vol. 35 ›› Issue (3): 503-506.

Skp2-P^27kip1通路及其相关凋亡基因在结肠癌组织中的表达及意义

陈树军^1,2, 郎晓讴², 张春阳², 张少军^1,2, 姜文华¹

1.吉林大学基础医学院组织学与胚胎学教研室|吉林长春130021； 2.沈阳医学院奉天医院普外科|辽宁沈阳110024

收稿日期:2009-03-04 出版日期:2009-05-28 发布日期:2009-08-14
通讯作者: 姜文华 E-mail:jiangwenhua468 @163.com
作者简介:陈树军(1970-),男,辽宁省沈阳市人,副主任医师,医学硕士,主要从事消化道肿瘤的病因学研究。
基金资助:
吉林省科技厅自然科学基金资助课题(20030542-1)

Expressions of Skp2-P^27kip1 and apoptosis-related genes in colon carcinoma and significances

CHEN Shu-jun^1,2,LANG Xiao-ou²,ZHANG Chun-yang²,ZHANG Shao-jun²|JIANG Wen-hua¹

1.Department of Histology and Embryology,School of Basic Medical Sciences,Jilin University,Changchun 130021| China； 2.Department of General Surgery,Fengtian Hospital,Shenyang Medical College,Shenyang 110024,China

Received:2009-03-04 Online:2009-05-28 Published:2009-08-14

摘要/Abstract

摘要：

目的：探讨Skp2和P^27kip1蛋白在结肠癌中的表达及其相关性，阐明两者在结肠癌的发生和发展中的作用。方法：应用免疫组织化学SABC法检测50例结肠癌患者癌组织和20例正常结肠组织中Skp2、P^27kip1、P53和Bcl-2蛋白的表达，比较不同组织中阳性细胞表达百分比,分析其与结肠癌临床病理参数的相关性。结果：与正常组织比较，结肠癌组织中Skp2蛋白阳性表达率增加（P<0.05），P^27kip1蛋白阳性表达率降低（P<0.05）。Skp2蛋白阳性表达率在临床Duck’s分期C和D期结肠癌、低分化结肠癌及伴有淋巴结转移的结肠癌组织中增加（P<0.05），P^27kip1蛋白阳性表达率在临床Duck’s分期A和B期结肠癌、高分化结肠癌及无淋巴结转移的结肠癌组织中增加（P<0.05）。P53 和Bcl-2蛋白在低分化结肠癌组织中均高表达（P<0.05）。结肠癌中Skp2与P^27kip1的表达率呈负相关关系（r=－0.282，P<0.05），与P53 及Bcl-2蛋白阳性表达率分别呈正相关关系（r1= 0.345，r1= 0.231，P<0.01）。结论： Skp2和P^27kip1蛋白的异常表达参与了结肠癌组织的分化、浸润和转移，相关凋亡基因P53 及Bcl-2蛋白表达上调可能是其作用机制之一。

Abstract:

To investigate the expression and correlation of Skp2 and P^27kip1 in colon carcinoma tissue and explore the relationship with the development of colon carcinoma.Methods The protein expressions of Skp2,P^27kip1,P53 and Bcl-2 were detected by immunohistochemistry staining (SABC method) in 50 cases of colon carcinoma tissue (CCT) and 20 normal colonic mucosas (NCM).The positive expression rates in different tissues were compared and the relationship between the expression and different clinicopathological parameters were analyzed.Results The expression of Skp2 in the CCT was significantly increased compared with NCM（P<0.05）.The protein expression of P^27kip1 in CCT was significantly lower than that in NCM（P<0.05）.The expressions of Skp2 were significantly increased in the Duke’s staging C and D group,lower degree differentiation group and lymph node metastases positive group（P<0.05）.The expressions of P^27kip1were significantly increased in the Duke’s staging A and B group,high degree differentiation group and lymph
node metastases negative group（P<0.05）.The expressions of P53 and Bcl-2 protein were significantly increased in the low degress differentiation CCT（P<0.05）. The negative correlation was present between Skp2 and P^27kip1 in CCT（r=－0.282，P<0.05）,and there were positive correlations between Skp2 and P53,Bcl-2 in CCT (r=0.345，r=0.231,P<0.05）.Conclusion The abnormal expressions of both Skp2 and P^27kip1 participate in the differentiation,infiltration and metastases of colon carcinoma. It may be realized through up-regulating the expressions of P53 and Bcl-2.

Key words: colon neoplasm；Skp2；P^27kip1；protein P53 ；proto-oncogene protein c-bcl-2

中图分类号:

R735.35

陈树军, 郎晓讴, 张春阳, 张少军, 姜文华. Skp2-P^27kip1通路及其相关凋亡基因在结肠癌组织中的表达及意义[J]. J4, 2009, 35(3): 503-506.

CHEN Shu-Jun, LANG Xiao-Ou, ZHANG Chun-Yang, ZHANG Shao-Jun, JIANG Wen-Hua. Expressions of Skp2-P^27kip1 and apoptosis-related genes in colon carcinoma and significances[J]. J4, 2009, 35(3): 503-506.