关键词: 骨髓间充质干细胞, 5-氮杂胞苷, 细胞增殖, 细胞分化

Abstract: Objective To investigate the influences of 5-azacytidine (5-Aza) with different concentrations on mesenchymal stem cells (MSCs) proliferation and differentiation into myoblasts. Methods Bone marrow-derived MSCs of 4 weeks Wistar rats were separated and purified,and then treated with 5-Aza with different concentrations. The growth ability of cell was assayed with methyl thiazolyl tetrazolium (MTT) method. The expression of skeletal muscle actin in MSCs was determined with reverse transcription polymerase chain reaction (RT-PCR) and electrophoresis after MSCs were induced.Results The cell proliferation was not affected by 0 and 1 μmo l·L^-1 5-Aza . There were expressions of skeletal muscle actin treated w ith 3-12 μmol·L^-1 5-Aza. Some cells were obviously enlarged at the 9th day after induction and myotubu-like cells were found at the 12th day when treated with 9 and 12 μmol·L^-1 5-Aza. 20-30 μmol·L^-1 5-Aza induced the toxic effect on proliferation of MSCs. With the increase of concentration, the proliferation ability of MSCs was weakened.Conclusion 5-Aza affects the expression of regulatory gene to the stem cells and regulate MSCs to orientationally differentiate into myotube- like cells.

Key words: mesenchymal stem cells, 5-azacytidine, cell proliferation, cell differentiation