关键词: 非小细胞肺, 微生物敏感性试验, 蛋白质p53, GST-π

Abstract: Objective To study the drug sensitivity of six kinds of chemotherapeutic drugs for human non-small cell lung cancer(NSCLC), and their relationships with the expressions of two drug-resistance factors p53 antibody and GST-π. Methods Fifty-eight NSCLC specimens obtained by operation were used for primary culture. Then the sensitivities of the cells to six kinds of chemotherapeutic drugs were detected by MTT assay. The serum GST-π and p53 antibody in the patients were tested by ELISA at the same time. Results The effective rates of the six kinds of drugs were Cisplatin (DDP) 41.4%, Vicristine (VCR) 24.1%,Etoposide (VP16) 22.4%, Hydroxyl-camptothecin (HCPT) 20.7%,Adriamycin (ADM) 17.2%,Cyclophosphamide (CTX) 17.2%,respectively. The patients had different multi-drug resistance when they had different positive expressions of p53 antibody and GST-π. Conclusion It is possible and necessary to test the drug sensitivity of lung cancer specimens obtained from operation by MTT method, and the results are very useful for the clinic chemotherapy after operation, especially when p53 antibody and/or GST-π positively express in the tissues.

Key words: non-small-cell lung, microbial sensitivity tests, p53 protein, GST-π