胡芦巴对糖尿病大鼠肾脏基质金属蛋白酶-2活性的影响

doi:吉林省科技厅科技发展计划重大项目资助课题

胡芦巴对糖尿病大鼠肾脏基质金属蛋白酶-2活性的影响

苗春生，石艳，于晓艳，李才

吉林大学药学院实验药理与毒理学教研室，吉林长春 130021)

收稿日期:2007-05-31 修回日期:1900-01-01 出版日期:2008-01-28 发布日期:2008-01-28
通讯作者: 李才

Effect of fenugreek seeds on renal MMP-2 activity in diabetic rats

MIAO Chun-sheng,SHI Yan,YU Xiao-yan,LI Cai

（Department of Experimental Pharmacology and Toxicology,School of Pharmacy,Jilin University,Changchun 130021,China）

Received:2007-05-31 Revised:1900-01-01 Online:2008-01-28 Published:2008-01-28
Contact: LI Cai

摘要/Abstract

摘要： 目的：探讨胡芦巴种子水煎剂（胡芦巴）对实验性糖尿病大鼠肾脏MMP-2活性的影响。方法：利用链脲佐菌素(STZ)制作糖尿病大鼠模型，造模成功大鼠随机分为糖尿病模型组（DM）10只和胡芦巴组（FN）10只，另设10只正常大鼠对照组（N）。FN组糖尿病大鼠给予胡芦巴12周后，观察各组肾脏结构和MMP-2活性的变化。结果：光镜观察给予胡芦巴处理12周后，与DM组比较FN组肾小球病变减轻。免疫组化检测DM组肾小球Col Ⅳ表达明显增强，FN组Col Ⅳ表达明显低于DM组。FN组MMP-2活性形式酶解量为（1.41±0.18），与DM组（1.05±0.19）比较明显增多（P<0.05）；与N组（1.53±0.25）比较差异无显著性。结论：胡芦巴可能通过增强肾脏MMP-2活性减轻实验性糖尿病大鼠肾脏病变的程度。

关键词: 胡芦巴, 药理学, 基质金属蛋白酶-2

Abstract: Abstract:Objective To explore the effect of boiled fenugreek seeds on renal matrix metalloproteinase-2(MMP-2) activity in diabetic rats. Methods The model of diabetes was built with STZ in rats.The model rats were randomly divided into diabetes control groups (DM ) （n=10） and fenugreek seeds groups(FN) （n=10,and while normal control group (N) （n=10）rats was used.Diabetic rats were treated with fenugreek seeds for 12 weeks,the renal morphology and MMP-2 activity were observed in three groups . Results After diabetic rats were treated with fenugreek seeds for 12 weeks，optical microscopic examination indicated that the glomerular structure in N group was normal,the glomerular lesions in rats of DM groups were seriously and the pathologic changes of glomerular in rats of FN groups were alleviated significantly.Immunohistochemical results showed that the Col Ⅳ expression in glomerular ECM was increased in DM group compared with N group,and was decreased in FN group.The activity of MMP-2 was increased in FN group (1.41±0.18) compared with DM group (1.05±0.19) (P<0.05) and the alterations were not manif ested compared with N group (1.53±0.25).Conclusion Fenugreek seeds may alleviate renal lesions through up-regulating MMP-2 activity.

Key words: Trigonella foenumgraecum, pharmacology, matrix metalloproteinase-2

中图分类号:

R285.5

苗春生，石艳，于晓艳，李才. 胡芦巴对糖尿病大鼠肾脏基质金属蛋白酶-2活性的影响[J]. J4, 2008, 34(1): 87-89.

MIAO Chun-sheng,SHI Yan,YU Xiao-yan,LI Cai. Effect of fenugreek seeds on renal MMP-2 activity in diabetic rats[J]. J4, 2008, 34(1): 87-89.

[1]	治丁铭, 隋殿军, 岂蕊, 苗永迪, 韩冬, 娄石磊, 邱悦, 孙聪. 基于网络药理学探讨葛根治疗高脂蛋白血症的潜在作用靶点[J]. 吉林大学学报(医学版), 2018, 44(04): 724-730.
[2]	汪佳琦, 姜文月, 张琳, 王君花, 赵芯, 王雨, 刘忠英. 胡芦巴中皂苷、黄酮和多糖组分不同配伍对1型糖尿病大鼠的降血糖作用[J]. 吉林大学学报(医学版), 2016, 42(06): 1081-1086.
[3]	沈楠, 陈钱, 齐玲, 陈雪, 黄成然, 唐海如, 安英, 林峰, 王艳春. 萱草花总黄酮含药血清对肝癌HepG2细胞增殖和凋亡的影响[J]. 吉林大学学报(医学版), 2016, 42(04): 737-741.
[4]	李慧敏, 李一雷, 宋雪霏, 董欣洁, 刘志晶, 梁珊珊, 张丽红, 李伟. 人子宫颈癌组织中MMP-26、MMP-9和TIMP-4的表达及其意义[J]. J4, 2010, 36(2): 354-358.
[5]	邢沈阳，乔萍，温得中，朱玉琢. 益母草提取液对小鼠遗传物质损伤的保护作用及对淋巴细胞功能的增强作用[J]. J4, 2008, 34(5): 799-801.
[6]	吕虹，刘莉，李欣欣. EMBASE数据库检索途径、检索方法及检索技巧[J]. J4, 2007, 33(4): 775-776.
[7]	刘青，郭蔚莹，刘克辉，谢晓娜，王丽颖. 重组人酸性成纤维细胞生长因子对糖尿病大鼠难愈性皮肤溃疡的促愈合作用[J]. J4, 2007, 33(1): 61-63.
[8]	卜凤泉，刘忠英，　石艳，　张静，苗春生，李才. 胡芦巴提取物对实验性糖尿病大鼠的降血糖作用[J]. J4, 2007, 33(1): 88-90.
[9]	石艳，李才，苗春生. 胡芦巴降血糖作用及其机制的研究进展[J]. J4, 2007, 33(1): 189-191.
[10]	胡翠华，徐华丽，于晓风，曲绍春，睢大员，陈燕萍，马兴元. 人参二醇组皂苷对实验性2型糖尿病大鼠血糖及血脂代谢的影响[J]. J4, 2006, 32(6): 1004-1008.
[11]	胡艳丽，王志祥，张梅，吴越，杨德林. 苯妥英锌对癫痫小鼠脑内氨基酸含量的影响[J]. J4, 2006, 32(6): 971-973.
[12]	李洋，曲绍春，于晓风，徐华丽，睢大员. 刺五加叶皂苷对麻醉犬器官血流的影响[J]. J4, 2006, 32(6): 1009-1011.
[13]	焦淑萍，倪海镜，杜培革. 山葡萄多酚对大鼠红细胞膜结构稳定性的影响[J]. J4, 2006, 32(5): 829-831.
[14]	1. 吉林大学口腔医院药剂科，吉林长春130041；2. 吉林大学口腔医院黏膜病科，吉林长春130041；3. 吉林大学第一医院口腔科，吉林长春130021. 化疗药物对舌癌细胞增殖能力的影响[J]. J4, 2006, 32(4): 672-674.
[15]	李健，李凤，庞爱芝，冯秀艳. 姜黄素对Hela细胞周期各时相的影响[J]. J4, 2006, 32(4): 675-677.