人脂肪干细胞向脂肪细胞的定向分化

J4 ›› 2009, Vol. 35 ›› Issue (6): 987-991.

人脂肪干细胞向脂肪细胞的定向分化

杨旭芳^1,2, 何旭¹, 何牮¹, 张丽红¹, 李玉林¹

（1.吉林大学基础医学院病理生物学教育部重点实验室|吉林长春 130021；2.牡丹江医学院病理生理学教研室|黑龙江牡丹江 157011 ）

收稿日期:2009-08-01 出版日期:2009-11-28 发布日期:2009-11-28
通讯作者: 李玉林 E-mail:（T李玉林(0431-85619481，E-mail： ylli@jlu.edu.cn）;何旭（Tel： 0431-85619481，E-mail： hexu00@163.com）
作者简介:杨旭芳（1974-）女|黑龙江省牡丹江市人|讲师|医学博士|主要从事干细胞组织工程学研究。
基金资助:
国家自然科学基金资助课题（30700872）；国家863重大专项基金资助课题(2004AA205020)；吉林大学青年教师基金资助课题(419070100050)

Differentiation of human adipose-derived stem cells into adipocytes

YANG Xu-Fang^1,2, HE Xu¹, HE Jian¹, ZHANG Li-Gong¹, LI Yu-Lin¹

1.Key Laboratory of Pathobiology,Ministry of Education,Jilin University|Changchun 130021|China；2.Department of Pathophysiology|Mudanjiang Medical College|Heilongjiang 157011|China）

Received:2009-08-01 Online:2009-11-28 Published:2009-11-28

摘要/Abstract

摘要：

目的：分离和培养人脂肪干细胞（hADSCs），并在体外定向诱导hADSCs分化为脂肪细胞。方法：利用胶原酶消化法和贴壁筛选法从人脂肪组织中分离、培养及扩增hADSCs；应用地塞米松、吲哚美辛、3-异丁基-1-甲基黄嘌呤及胰岛素定向诱导hADSCs分化为脂肪细胞。结果：体外分离、培养出高度同源性的hADSCs，hADSCs具有相对特异的细胞表面标志，即CD29、CD73、CD105及CD166呈阳性表达，而CD31及HLA-DR呈阴性表达；hADSCs具有典型的干细胞增殖特点，大部分处于静息期，只有少数细胞处于增殖活跃期；hADSCs能被诱导分化为脂肪细胞。结论：成功地分离、培养出高度同源性的、未分化的hADSCs；体外定向诱导hADSCs可分化为脂肪细胞。

关键词: 干细胞；流式细胞术；脂肪细胞

Abstract:

To find the most effective way to obtain highly homogenous and undifferentiated human adipose-derived mesenchymal stem cells（hADSCs）,and induce the hADSCs to differentiate into adipocytes.Methods hADSCs were isolated from human adipose tissue by collagenase digestion and adherence to flasks.hADSCs were differentiated into adipocytes by using dexamethasone,indomethacin,IBMX and insulin.Results hADSCs had unique immunophenotypes and they were positive for CD73,CD44,CD166,CD105 and CD29,but negative for CD31,CD34,CD45 and HLA-DR.hADSCs had the typical proliferative characteristics of stem cells，most of them were in the resting phase ，only a few cells were in the active proliferative phase.They could be induced to differentiate into adipocytes.Conclusion It is successful to isolate and obtain highly homologous hADSCs,and it was also successful in inducing the hADSCs to differentiate into adipocytes in vitro.

Key words: stem cells, flow cytometry, adipocytes

中图分类号:

Q813.1

杨旭芳, 何旭, 何牮, 张丽红, 李玉林. 人脂肪干细胞向脂肪细胞的定向分化[J]. J4, 2009, 35(6): 987-991.

YANG Xu-Fang, HE Xu, HE Jian, ZHANG Li-Gong, LI Yu-Lin. Differentiation of human adipose-derived stem cells into adipocytes[J]. J4, 2009, 35(6): 987-991.