人激肽释放酶10在卵巢癌组织中的表达及其意义

doi:吉林省科技厅基金资助课题(200705155);

人激肽释放酶10在卵巢癌组织中的表达及其意义

张晓霞¹，王影¹，黄蓉¹，李相军²，任立群²，李荷莲³

1.吉林大学第一医院妇产科，吉林长春 130021； 2.吉林大学药学院实验药理与毒理学教研室，吉林长春 130021;3.吉林大学第二医院妇产科，吉林长春 130041

收稿日期:2008-02-25 修回日期:1900-01-01 出版日期:2008-07-28 发布日期:2008-07-28
通讯作者: 李荷莲

Expression of hK10 in ovarian cancer tissue and its significances

ZHANG Xiao-xia¹,WANG Ying¹,HUANG Rong¹,LI Xiang-jun²,REN Li-qun²,LI He-lian³

1.Department of Obstetrics and Gynecology,First Hospital,Jilin University,Changchun 130021,China;2. Department of Experimental Pharmacology and Toxicology,School of Pharmacy,Jilin University,Changchun 130021,China;3.Department of Obstetrics and Gynecology,Second Hospital,Jilin University,Changchun 130041,China

Received:2008-02-25 Revised:1900-01-01 Online:2008-07-28 Published:2008-07-28
Contact: LI He-lian

摘要/Abstract

摘要： 目的：研究人激肽释放酶10（hK10）在卵巢癌组织中的表达，探讨hK10基因在卵巢癌早期诊断研究中的意义。方法：①应用4 097靶基因点基因芯片对5例卵巢浆液性囊腺癌及5例正常卵巢组织进行基因表达谱分析，筛查差异表达基因。②应用RT-PCR检测32例卵巢浆液性囊腺癌组织、20例卵巢浆液性囊腺瘤组织及16例正常卵巢组织中hK10表达并对比分析。结果：①基因芯片筛查出差异表达基因58个，其中表达增高（上调趋势）基因30个，表达降低（下调趋势）基因28个，hK10基因为表达上调基因（Cy5/Cy3＝3.031）。②hK10基因在正常卵巢及卵巢瘤组织中有弱表达(0.023±0015和0.023±0.045)，在卵巢癌组织中表达明显增强(0.106±0.045)，hK10基因在卵巢癌组的表达明显高于正常卵巢组及卵巢瘤组（P<0.01）。结论：基因芯片能够用于筛查卵巢癌差异表达基因；hK10基因在卵巢癌组织表达明显增强，有望成为卵巢癌的肿瘤标志物。 

关键词: 人激肽释放酶10, 基因芯片, 逆转录聚合酶链反应

Abstract: Objective To study the expression of hK10 in ovarian cancer tissue and the significances of hk10 gene in the early diagonosis of ovarian cancer. Methods ①cDNA microarray containing 4 097 genes was used to analyze gene expression patterns in samples from 5 cases of human ovarian serous cystadenocarinoma and 5 cases of normal ovarian tissues. ②RT-PCR method was used to investigate the expressions of hK10 mRNA in samples of 32 serous cystadenocarinoma,20 serous cystadenoma and 16 normal ovarian tissues. Results ①58 genes of differential expression were found in ovarian cancer tissue by cDNA microarray. The expression of 30 genes increased (up-regulated),the expression of 28 genes decreased (down-regulated). hk10 was the up-regulated gene（Cy5/Cy3＝3.031）. ②The expression levels of hK10 gene in normal ovarian tissues and benign ovarian tumor tissues were lower,but higher in ovarian cancer tissues.The expression level of hK10 was 0.023±0.015 in normal ovarian tissues,0.032±0.045 in benign ovarian tumor tissues and 0.106±0.045 in ovarian cancer tissues.The expression level of hK10 gene in ovarian cancer tissues was significantly higher than those in normal ovarian and benign ovarian tumor（P<0.01）. Conclusion cDNA microarray could be used to screen differential expression gene in ovarian cancer;the expression level of hK10 gene is increased in ovarian cancer tissues,it may be a new tumor marker for ovarian cancer.

Key words: hK10, gene microarray, reverse transcription polymerase chain reaction

中图分类号:

R737.33

张晓霞，王影，黄蓉，李相军，任立群，李荷莲. 人激肽释放酶10在卵巢癌组织中的表达及其意义[J]. J4, 2008, 34(4): 661-664.

ZHANG Xiao-xia,WANG Ying,HUANG Rong,LI Xiang-jun,REN Li-qun,LI He-lian. Expression of hK10 in ovarian cancer tissue and its significances[J]. J4, 2008, 34(4): 661-664.

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