Abstract:

Objective
To investigate the expressions  of platelet-derived growth factors(PDGFs) in primary adenocarcinoma of stomach tissues,and clarify their relationships with clinicolpathological features of primary adenocarcinoma of stomach. Methods The mRNA expressions  of PDGF-A，B，C，D were assessed by reverse transcription-polymerase chain reaction（RT-PCR）in 58 cases of gastric cancer and adjacent carcinoma tissues. Immunohistochemical staining was used to examine the expression of PDGF-D protein in gastric cancer and adjacent normal tissues. Results The positive expression rates of PDGF-A,B,C,D mRNA were 39.66%,22.41％,31.03%, and 98.28％ in gastric cancer tissues and 12.07％,10.34%, 17.24%, and 72.41% in adjacent normal tissues,respectively.The positive  expression rate of PDGF-D mRNA  in gastric cancer tissues was significantly higher than that in adjacent normal tissues（P<0.05）.The expression of PDGF-D mRNA was significantly higher than other PDGF mumbers in gastric cancer tissues (P<0.0１).The PDGF-D protein was present in membrane/ cytoplasm and the difference between gastric cancer and adjacent normal tissue was statistically significant (P<0.05). The PDGF-D mRNA expressions in groups with lymph-node metastasis,distant metastasis and relapse were higher than those in the groups without metastasis and relapse (P<0.05）. Conclusion PDGF-D is the most important mumber of PDGFs and has a close relationship with human gastric cancer. It can be used as a prognostic marker of gastric cancer in clinical practice.

Key words: platelet-derived growth factors;stomach neoplasms;reverse transcriptase polymerase chain reaction;immunohistochemistry