Abstract:

Abstract:Objective
To screen the high chitinase-producing fungi from the samples in the environment,and investigate the inhibitory effect  of the enzymes from the strains on  the Candida  for the development of antifungal drug.Methods  Congo red-chitin plates and classical  DNS method were used to screen the high chitinase-producing strains,and  the filter paper containing the chitinase was used to detect the inhibitory effect of the enzymes.Results 6 chitinase-producing strains were screened from 47 strains.  2 high chitinase-producing strains（Aspergillus fumigatus JLC 50134 and Trichoderma sp.JLC 31235）were obtained. The enzyme from JLC 50134 had better inhibitory effect  on Candida albicans and Candida tropicalis,and the individual diameters of the inhibition zone were 4-5 cm and about 2 cm;while the enzyme from JLC 31235 had better inhibitory effect on Candida krusei and Candida glabrata than JLC 50134,and the individual diameters of the inhibition zone were about 2 cm and 1 cm. Conclusion High chitinase-producing strains (JLC 50134 and JLC 31235) are obtained ,and they have different inhibitiory effects on Candida.

Key words: fungi;chitinase;Candida