Abstract:

Abstract:Objective To establish an ovarian cancer cells with high expression of LPA1 by transfecting LPA1 genes into the ovarian carcer cell lines and to study thebiological characteristics of the transfected cells and to study the effects of lysophosphatidic acid (LPA) on the expressions and activities of MMP-2 and MMP-9 inovarian cancer cells with stable expression LPA1 genes.Methods The pcDNA3.1-LPA1 was transfected into ovarian cancer cells line (SKOV3 and 3AO)with lipofectaminereagent.Control and vector groups were set up.Transfection and expression were confirmed by the means of RT-PCR.The biological characteristics of the transfected cellswith expression LPA receptor genes were studied by the observation of their growth and proliferative period.The effects of LPA on the of MMP-2 and MMP-9 protein expressions and activities in transfected ovarian cells were analyzed by gelatin zymography.Results It was confirmed that the LPA1 genes had been stably integrated into 3AO and SKOV3 cell lines.The growth characteristics of transfected cells were not different before and after transfectation.The high expression of LPA1 gene was revealed by the means of RT-PCR.The expression levels of LPA1 mRNA in transfected cells （SKOV3-LPA1 and 3AO-LPA1） were significantly higher than those in control transfected cells and an empty vector groups,there were significant differences (P<0.05).The activities and protein expressions of MMPs in the cells  transfected with LPA1 receptor genes did not increase,compared with control and empty vector groups (P>0.05).Conclusion The ovarian carcer cells with high expression of LPA1 gene in vitro have been established.LPA1 plays a role as negative growth receptor in the process of LPA-induced MMPs invasion and metastasis of ovarian carcer cells.

Key words: lysophosphatidic acid recepor;matrix metalloproteinase;ovarian neoplasms