Abstract:

Abstract:Objective To study the inhibitory effects of melatonin(MLT),cisplatin(DDP),MLT combined with DDP on human ovarian cancer cell line SKOV3,and provide the theoretical basis for the study on MLT as an ancillary drug of ovarian cancer. Methods The ovarian cancer cell SKOV3 at logarithmic growth phase were divided into control group,MLT groups(0.5,1.0 and 2.5 mmol/L),DDP groups(25,50 mg/L) and MLT combined with DDP groups(MLT 0.5 mmol/L combined with DDP 25 mg/L；MLT 0.5 mmol/L combined with DDP 50 mg/L；MLT 1.0 mmol/L combined with DDP 25 mg/L；MLT 1.0 mmol/L combined with DDP 50 mg/L；MLT 2.5 mmol/L combined with DDP 25 mg/L；MLT 2.5 mmol/L combined with DDP 50 mg/L). The apoptotic rate and proliferation inhibitory rate of ovarian cells were detected with microscopic observation and MTT assay,and the expression of VEGF in ovarian cancer cell SKOV3 was determined. Results Observing with microscope,in the cells treated with drugs the adherence ability was reduced with refraction,shrink,circular,karyopycnosis,nuclear fragmentation and the cell chromatin agglutination. The apoptotic cells of solo drug groups were more than those in combined drug groups. MTT assay indicated that the MLT groups(0.5,1.0,2.5 mmol/L) and MLT combined with DDP(25,50 mg/L) groups all had inhibitory effects on the cell growth,and the inhibitory rate was increased with the concentrations of drugs. Two drugs had synergetic effect,CDI＜1. Immunocytochemical staining results showed that the expressions of VEGF in solo drug groups and combined groups were lower than that in control groups,the expression of VEGF in combined groups was decreased significantly(P<0.05). Conclusion  MLT can restrain the growth of ovarian cancer cells. MLT combined with DDP has synergetic effect on down-regulation of the expression of VEGF. Combined DDP with debita spissitudine MLT can achieve good curative effect.

Key words:  Ovarian neoplasms；vascular endothelial growth factor；melatonin； cisplatin；cell immunohistochemical