Abstract:

Abstract:Objective To observe the structure changes of the synapse of the neurons in hippocampus CA3 of young  rats and study the basis for the mechanism of polyene phosphatidyl in providing learning and memory and the effect on  synaptic plasticity. Methods A total of 20 Wistar rats with 5 months were randomly divided into polyene phosphatidyl group and normal control group. Each group had 10 rats. After 4 weeks feeding, Water maze training was peformed in all the rats for 1 weeks. The immue expressions of synapsin (SYN) of the rats in polyene phosphatidyl group  and  control groups  were observed with immunohistochemical method and analyzed by MOTAC imagine analyzing system. The change of synapse of hippocamal CA3 was observed with electron microscope. And  the other 24 to 26 months rats were selected as aged group,and  fed in the same condition. Moreover, the ultrastructures of hippocamal CA3 of aged rats were observed by transmission electron microscopy(TEM).Results The SYN in  polyene phosphatidyl group(0.430 0±0.022 4) was higher than that in  control group(0.3567±0.0209)  (P<0.05).  The density of SYN in polyene phosphatidy1 group was (102.69±8.96)•μm^-3 and the area of SYN membrane in the region of hippocamal CA3 was (0.066±0.010) μm²/μm³, which were higher than those in  control group(82.89•μm^-3±6.52•μm^-3, .046μm²/μm³±0.006 μm²/μm³ ) (P<0.05). Under TEM the  abundance of synapse had complete structure, the synapse cleft were clear and the spine apparatus were active. The synaptic vesicles in aged rats increased, the size was different, and accumulation presented. The spine apparatus were far away from postsynaptic membrane; the flat vesicles of the spine apparatus were expanded and increased in number.Conclusion Additive polyene phosphatidyl  can improve the expression  of SYN  in the region of hippocamal CA3 and  increase the density of SYN and the area of SYN membrane,  and improve  the special discrimination of learning and memory in young rats.

Key words: polyene phosphatidyl, hippocamal CA3, synapse, synaptophysia, synaptic plasticity