Abstract:

Objective
To explore the effect of ginsenoside Rb1 on hippocampal neuron injury after treated by Aβ-amyloid protein in rats and provide theoretical basis for its  clinic application.Methods The cultured hippocampal neurons from newborn SD rats were divided into normal control group,hippocampal neurons treated with Aβ-amyloid protein group (injury control group),low dose(10 mg/L) Rb1 group,middle dose(50 mg/L) Rb1 group, and high dose(100 mg/L) Rb1 group.Then the  survival rates of neurons were detected by MTT colorimetry and the apoptosis of neurons were detected by TUNEL staining，and the caspase-3 activities of neurons were detected by immunohistochemistry method.Results The MTT results showed that the survial rates  of hippocampal neurons were decreased in injury control  group,10 mg/LRb1 group,50 mg/LRb1 group and 100 mg/L Rb1 group  compared with normal control group(P<0.05).Compared with injury control group,the survial rates  in 50 mg/L Rb1 group and 100 mg/L Rb1 group were increased(P<0.05).The TUNEL results  showed that compared with normal control group,the apoptosis of neurons  in  injury control group,10 mg/L  Rb1 group,50 mg?L-1 Rb1 group and 100 mg?L-1 Rb1 group were increased(P<0.05).The aproptotic rate  of neurons in 100 mg/L Rb1 group was higher than that in  injury control group (P<0.05).The expression of caspase-3  in    100 mg/L Rb1 group was lower than that in injury control  group (P<0.05).Conclusion Ginsenosides Rb1 can protect hippocampal neurons against Aβ-amyloid protein injury,which is achieved through the regulation of apoptosis.

Key words: ginsenoside Rb1；hippocampal neurons；caspase-3；apoptosis