Abstract:

To investigate the regulatory role of E6-associated protein(E6-AP) in anadrogen receptor(AR) and AKT expressions in prostate cancer LNCaP cells and its mechanism. Methods  LNCaP cells stably transfected with E6-AP were used to analyze the regulatory role of E6-AP in AR and AKT expressions by Western blotting assay,the  intracellular localization of E6-AP and AR was determined by immunofluorescence. Results Compared with LNCap cells with low E6-AP expression, the total AR and phosphorylated-AR expression levels in the  LNCaP cells with high E6-AP expression were significantly decreased（P<0.05）,the  total Akt and phosphorylated-Akt expression levels were significantly increased（P<0.01）.The immunofluorescence results demonstrated that E6-AP showed a similar intracellular location with AR in the LNCaP cells.  Conclusion Overexpression of E6-AP could down-regulate the protein level of total AR and phosphorylated-AR,whereas up-regulate the protein level of total Akt and phosphorylated-Akt. E6-AP shows colocalization with AR in the LNCaP cells.

Key words: androgen receptor；E6-associated protein；serine/threonine protein kinase