Abstract:

Objective To investigate the interventional effect of oxymatrine (OMT) on focal cerebral ischemia and explore the mechanism of its intervention on oxidative stress response.Methods The Wistar rats were randomly divided into sham operation group,model group,positive drug group,OMT1 (35 mg/kg) group,OMT2 (70 mg/kg)　 group and OMT3 (105 mg/kg) group (n=10).The drug was continuously administrated 5 d before the surgery of middle cerebral artery occlusion (MCAO) was carried out.The neurological score was recorded 24 h after operation and the  morphological changes of ischemic brain tissue were observed by HE staining.The interventional effects of OMT on oxidative stress of cerebral ischemic rats were investigated by measuring the activities of superoxide dismutase (SOD),catalase (CAT) and glutathione-SH peroxydase (GSH-Px) and the content of malondialdehyde (MDA) in peripheral serum.Results Compared with model group,the neurological scores of rats in OMT groups were significantly decreased(P<0.05 or P<0.01),the morphological characteristics of ischemic brain tissue were maintained and  the damage of tissue  was alleviated with a certain degree;the  activities  of SOD,CAT and GSH-Px were increased (P<0.05 or P<0.01)　and  the contents of MDA reduced (P<0.05 or P<0.01)  with a  dose-effect relationship.Conclusion OMT can intervene the focal cerebral ischemic injury by influencing the oxidative stress respone in rats with cerebral ischemic injury.

Key words: oxymatrine;cerebral ischemia;oxidative stress response;superoxide dismutase;catalase;malondialdehyde