Abstract:

Abstract:Objective
To establish the method to induce human bone mesenchymal stem cells(hMSCs) to differentiate into cardiomyocytes in vitro. Methods  hMSCs were isolated and purified from the bone marrow of human by density gradient centrifugation and adhering to the culture dishes.The fourth passage of MSCs was induced by 5-azacytidine(5-Aza) and bFGF for four weeks.The expression of Desmin,Troponin I and α-sarcomeric actin were detected by immunocytochemistry 3-4 weeks after induction.Results
hMSCs treated with 5-Aza and bFGF had long cytoplasmic process  one week  after induction,and had multiple branches  two weeks after induction,and they connected with adjoining cells forming myotube-like structures  four weeks after induction. The induced hMSCs were stained positively for Desmin,Troponin I and α-sarcomeric actin.Conclusion  5-Aza and bFGF can induce hMSCs to differentiate into cardiomyocytes in vitro.

Key words: mesenchymal stem cells;myocytes,cardiac;　5-azacytidine