Abstract:

Abstract:Objective
 To study the proteomic differences between malignant melanoma tissue and normal skin tissue and to look for the possible marker for diagnosis and therapy target of malignant melanoma. Methods Three samples of malignant melanoma tissues were ascertained by pathologic test. The total protein was extracted,separated with the method of 2D-DIGE; the difference of the proteomics was analyzed with DeCyder software; the differential-expressed protein spots were identified with MALDI-TOF/TOF-MS．Results A total of 12 protein spots were identified and categorized into 4 classes according to the function including 3 kinds of  up-regulated proteins (14-3-3 protein sigma,keratin and serpin B3)，and 1 kind of down-regulated protein (album)．Conclusion 14-3-3 protein sigma and serpin B3 are possible involved in the occurance and developmentof malignant melanoma. The mechanism of album change in the tissue of malignant melanoma remains unclear. The change of those four proteins suggests the possible pathogenesis of malignant melanoma.

Key words: malignant melanoma, proteomics;matrix-assisted laser desorption/ionization-time of flight-time of flight-mass spectrometry