J4 ›› 2011, Vol. 37 ›› Issue (4): 651-654.
Previous Articles Next Articles
JIANG Ya-ping1,SONG Wen-zhi1,YIN Wan-zhong2,YANG Huan1,ZHANG Tian-fu1,WANG Wei1,LI Mei-hua1
Received:
Online:
Published:
Abstract:
Abstract:Objective To investigate the cytotoxicity of gold nano-flower particle which has the absorption ability in near-infrared (NIR) region to human fibroblasts(HF) in vitro,and to provide basis for its application in biomedical field.Methods HF were cultured with medium contained different doses of gold nano-flower particles (1.875,3.750,7.500,15.000,30.000 mg·L-1) for 24 h,the HF cultured with medium free of nanoparticles as control group,the cytotoxicity effect was tested by MTT method,the morphology of HF was observed under inverted microscope.Results The relative growth rates(RGR)were 92.245%,88.980%,86.939%,75.782% and 71.756% respectively in different doses of gold nano-flower particle groups,the RGR in 30.000 mg·L-1 group had significant difference compared with control group(P<0.05),while all groups were the first cytoxicity grade.The HF had normal morphology and well growth under inver ted microscope observation in low dose gold nano-flower particle group.Conclusion The cytotoxicity of the gold nano-flower particles has does-effect response,and low dose of gold nano-flower particles have well biocompatibility with HF.
Key words: gold nano-flower;human fibroblast;cytotoxicity;MTT method;near-infrared
CLC Number:
JIANG Ya-ping,SONG Wen-zhi,YIN Wan-zhong,YANG Huan,ZHANGTian-fu,WANG Wei. Cytotoxicity of gold nano-flower particles to human fibroblasts in vitro[J].J4, 2011, 37(4): 651-654.
0 / / Recommend
Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks
URL: http://xuebao.jlu.edu.cn/yxb/EN/
http://xuebao.jlu.edu.cn/yxb/EN/Y2011/V37/I4/651
Cited