Abstract:

Abstract:Objective To investigate the effect of tripterygium wilfordii hoff-F on acute immune rejection of bone xenograft in mice.Methods Male Neijiang swine and BALB/c mice were used as donor and receipts,respectively.Forty mice were divided into experimental group and control group at random.The mice in experimental group were administered intragastrially with tripterygium wilfordii hoof-F before bone xenograft.At two weeks postoperatively,CD4⁺,CD8⁺,CD4⁺/CD8⁺T lymphocytes in spleen were detected by flow cytometry,and the levels of IL-2,IFN-γ and IL-4 in blood were analyzed by quantitative ELISA assay.And the infiltration of lymphocytes and macrophages surrounding soft tissues of bone graft was also observed in order to evaluate the immunosuppression of tripterygium wilfordii hoof-F.
Results At two weeks postoperatively,compared with control group,CD4⁺,CD8⁺,CD4⁺/CD8⁺T lymphocytes in spleen,the levels of IL-2,IFN-γ and IL-4 in blood of mice in  experimental group were significantly decreased(P<0.05).There was few lymphocytes infiltration surrounding soft tissue of bone graft in experimental group.

Key words: tripterygium wilfordii hoff-F, transplantation,heterologous, bones, immune rejection