Abstract:

Abstract:Objective To study the effect of 20(s)-proto-panaxdiol（PPD）on apoptosis of Siha cells in vitro,and clarify the mechanism of its anticancer action. Methods Siha cells cultivated in vitro were treated with alcohol,40 μg/L Platinol(Pt),10 μmol/L PPD,20 μmol/L PPD,40 μmol/L PPD for 48 h,respectively.The morphological changes of Siha cells were observed under optical microscope.Apoptosis was detected by Annexin V-EGFP /PI staining and TUNUL staining.The transcription and the protein expression of bax were analyzed by RT-PCR and Western blotting.Results After treated with PPD,the cells were round,recovery,and broken.Green and red fluorescence was shown in Annexin V-EGFP/PI staining,green fluorescence was evident in TUNUL staining.The transcription and the protein expression of bax were up-regulated.Conclusion PPD may induce the apoptosis of Siha cells,and its mechanism may be related to the up-regulation of bax expression.

Key words: 20(s)-protopanaxd aol;uterine cervical , neoplasms;Siha cells;apoptosis