Abstract:

Abstract:Objective To investigate the effects of E6-AP on cell proliferation and cell cycle of human prostate cancer cells and elucidate the role of E6-AP participating in androgen receptor signal pathway and in the progress of prostate cancer.  Methods tTA and E6-AP overexpression LNCaP cell line was established by electroporation transfection. The cell viability was analyzed by MTT assay and cell cycle distribution was detected by flow cytometry after propidium iodide staining using the stable cell line under the regulation of Dox. Results LNCaP cell line overexpressing E6-AP was established successfully. Compared with untreated LNCaP cell and Dox treated E6-AP stable cell line,the total number and the proportion in S phase of Dox treated E6-AP stable cells were increased significantly（P<0.01）. Conclusion Overexpression of E6-AP induces proliferation of LNCaP cells and increase the number of cells in S phase,indicating that E6-AP is involved in androgen receptor signal pathway.

Key words: E6-associated protein;prostatic neoplasms;cell proliferation;cell cycle