Abstract:

Abstract:Objective To investigate the effects of polyi:c on angiogenesis in mouse prostate carcinoma tissue and their possible mechanisms.Methods Mouse prostate carcinoma models were randomly divided into two groups according to tumor weight:control group and polyi:c group.After  treatment for 7 times,the mice were sacrificed and the tumor tissues were cut for weighing,calculating the tumor inhibitory rate and tumor index.Hematoxylin-eosin staining and immunohistochemical staining were performed to observate the morphological changes of prostate carcinoma tissues,distribution of vasa and expressions of vascular endothelial growth factor (VEGF) and endothelial nitric-oxide synthase (eNOS).
Results In polyi:c group,the mean tumor inhibitory rate was 67.85% and the tumor index was（5.42±0.17）%;in control group,the tumor index was（14.45±1.06）%;  there was significant differences between polyi:c group and control group( P<0.01). The expressions of VEGF in polyi:c group and control group were (9.64±2.90)% and (37.91±7.62)%, respectively,and there was significant difference between two groups( P<0.01). The expressions of eNOS in polyi:c group and control group were (4.43±10.39)% and (22.00±8.07)%，respectively,and there was significant difference between two groups ( P<0.01).Conclusion Through decreasing the expressions of VEGF
and eNOS,polyi:c plays an important role in inhibitting the angiogenesis and indirect inhibiting mouse prostate carcinoma.

Key words: polvriboinsine-polyribocyaidylic acid;prostaic neoplasms;vascular endothelial growth factor;endothelial nitric-oxide synthase