Abstract:

Abstract:Objective To observe the intestine absorption kinetics of berberine (Ber) in rats and the effects of sodium caprate(SC) on Ber in intestinal absorption. Methods Male Wistar rats were used for the experiment. The in situ circular perfusion model was used to investigate the effect of SC on the intestinal absorption of Ber. The concentration of Ber was detected with  HPLC.The  activity of LDH was measured to evaluate the toxic effect of SC on mucous membrane cells. Results The absorption of Ber in the small intestine was poor. The uptake of Ber presented dose-dependent manner. There was no significant difference on Ka among these groups (P>0.05). SC could significantly enhance the absorption amount of Ber in the small intestine (P<0.05). SC showed no significant effect on Ka of Ber(P>0.05). The LDH activities in circulating fluid  in Ber and SC+Ber groups  had no significant difference compared with control group (P>0.05). Conclusion The absorption of Ber in the small intestine is poor and complied with the passive diffusion mechanism and first order kinetics. SC can significantly enhance the absorption of Ber in the small intestine and is a safe,effective absorption accelerator.

Key words: sodium caprate;berberine;rats,Wistar;intestine;absorption kinetics