Abstract:

Abstract:Objective
To investigate the expression level of glucose regulating protein 78(GRP78) in SH-SY5Y cells in the Parkinson’s disease(PD)  model induced by dopamine(DA)，and provide the theoretical basis for PD．Methods PD models  induced by DA in SH-SY5Y cells were established.The cells in experimental group were treated with DA,and the cells in control group were treated with  equal volume of 0.2% vitamin C.The cells in two groups were incubated for 24 h, the viability of SH-SY5Y cells was assayed by Trypan blue staining,Hoechst 33342 fluorescence staining was used to estimate the apoptotic percentage．The proteins of SHSY5Y cells treated with or without DA were extracted 24 h after incubation，and then the maps of the proteins were established by 2D-DIGE(differential gel electrophoresis,DIGE).The altered protein spots were identified with MALDI-TOF MS and database searching.Results The survial rate of SH-SY5Y cells  in experimental group(53.2%) was lower than that in control group(100%)(P< 0.05)．The Hoechst fluorescence staining result showed that the nuclei of SH-SY5Y cells in exprerimental group appeared karyopyknosis and fragmentation which was consistent with nuclear morphology.A high expression protein spot in experimental group was confirmed as GRP78 by MALDI-TOF MS． Conclusion  DA can induce the apoptosis of SH-SY5Y cells,and the expression of GRP78 is increased in the process of apoptosis．The results demonstrate that the increase of GRP78 may be involved in the pathogenesis of PD．

Key words: S-SY5Y cell； dopamine； GRP78； differentialgel eletrophoresis