Abstract:

Abstract:Objective To study the protective effects of gross saponins from tribulus terrestris (GSTT) on ischemia-reperfusion(I/R) injury in isolated rat heart and approach its mechanism of action.Methods Fifty-six Wistar rats were randomly divided into seven groups:control group,I/R group,ischemic preconditioning(IPC) group,adenosine group,and GSTT 200,100,50 mg?L-1 groups.The isolated hearts were subjected to 30 min ischemia and then followed by 40 min reperfusion.The contents of CK,LDH,AST，MDA and activity of  SOD in reperfused ischemic rat heart were detected.The histopathological changes of myocardium were observed by hematoxylin-eosin (HE) staining.Results Conpared with I/R group,the contents of CK,LDH,AST were decreased in GSTT 200 and 100 mg?L-1 groups（P<0.05  and P<0.01），and the contents of MDA in myocardium tissues were also decreased（P<0.05 and P<0.01）,and the activities of SOD were increased（P<0.05 and P<0.01）.GSTT could  alleviate the pathological lesion of myocardium tissues.Conclusion GSTT has protective effect on cadiocytes injured by ischemia-reperfusion,its mechanism may be concerned with resisting oxygen free radical.

Key words: gross saponins from tribulus terrestris;myocardial ischemia;reperfusion injury；ischemic preconditioning；pharmacological preconditioning；myocardial enzyme;reactive oxygen species