Abstract:

Abstract:Objective To study the expression of the  recombinant protein of keratinocyte growth factor -2 (KGF-2)  induced with  lactose,an alternative to IPTG induction agents,and  explore the possibility of the production of KGF-2 engineering. Methods The effects of the concentration,induction time and induction temperature of lactose  on the  expression  of the recombinant product were compared and  IPTG induction was used as a control to determine a more optimal conditions for lactose induction. Results For the production of recombinant KGF-2,lactose as an induction agent could also play a very good role;the A600 value  with lactose as induction agent was   higher than  IPTG, the expression level of induction product was equivanlent to IPTG induction. With 10　g/L lactose,at 33℃,induction for  6 h,the  induction effect was  better and the induction product  had good solubility and  could promote the cell growth. Conclusion Lactose can replace IPTG to express the  keratinocyte growth factor,and the  good effects are obtained.This study  provides a good experimental basis for the large production of KGF-2.

Key words: keratinocyte growth factor -2;lactose induction;IPTG