Abstract:

Abstract:Objective
To investigate the expressions of Notch signal molecules and their downstream target genes in human NK/,NK,T and B lymphoma cells and to expound the effect and significance of Notch signal molecules in NK cell tumor.Methods The expression levels of Notch signal  in YTS,SNK-6,Raji and Jurkat cells were detected  by Real-time PCR.Results The expression levels of Notch 1,3,4 signal molecules in YTS and SNK-6 cells were significantly higher than those in Raji and Jurkat cells（P<0.01）,but the expression levels of Notch 1,3,4 signal molecules in SNK-6 cells were lower than that in YTS cells(P<0.01).The expression levels of  Notch 2  in YTS and SNK-6 cells were significantly higher than that in Jurkat cells（P<0.01）,but there was  no  statistically significant difference compared  with  Raji cells （P>0.05）.The expression level of Hes1 in YTS cells was significantly higher than those in Raji and Jurkat cells（P<0.05 or P<0.01）,but the expression level in SNK-6 cells was lower than those in Raji and Jurkat cells（P<0.01）.Conclusion Notch signal molecules  express abnormally in   NK cell tumor,and they are significantly different with B and T lymphoma cells.

Key words: Notch signal pathway, NK/T lymphoma, B lymphoma, T lymphoma, Rea-l time PCR