Abstract:

Abstract:Objective To discuss the effect of cyclooxegenase-2（COX-2） selective suppressor celecoxib on the expressions of vascular endothelial growth factor(VEGF) mRNA and matrix metalloproteinases-9(MMP-9) mRNA in human medullary thyroid TT cells,and to approach the anticancer mechanism of celecoxib.Methods  The TT cells of human medullary thyroid carcinoma were divided into control group and different concertrations of celecoxib groups,and the cells in celecoxib groups were treated with different concentrations  of celecoxib（20,40 and 80  μmol?L-1）,the cells in control group were treated with  F12K culture fluid.The expressions of VEGF mRNA and MMP-9 mRNA in each group were detected by RT-PCR 72 h after treatment. Results the RT-PCR results showed that the expressions of VEGF mRNA and MMP-9 mRNA in  TT cells were decreased  after  treated with  20,40 and 80  μmol?L-1 celecoxib. There was  significance difference of the VEGF/GAPDH and MMP-9/GAPDH of TT cells between 40,80  μmol?L-1 celecoxib groups and   control group（P<0.05）.Conclusion The secretion of VEGF mRNA and MMP-9 mRNA is decreased obviously when the concentration  of celecoxib is above 40  μmol?L-1,which may inhibit the growth of tumor.

Key words: thyroid neoplasms;celecoxib;vascular endothelial growth factor;matrix metalloproteinases-9