Abstract:

Abstract:Objective To observe the changes of the levels of serum sex hormones in mice treated with atrazine (ATR), and to evaluate the effect of  ATR on sex hormone related diseases. Methods 32 healthy female C57 mice were randomly divided into control group and 5, 25,and 125 mg/kg ATR groups;there were 8 mice in  each group.The mice in control group were given olive oil by gavage,and the mice in the other groups were given 5,25, and 125 mg/kg ATR once per day for 28 d. The serum of mice  were collected. The levels  of serum sex hormones  were detected by radioimmunoassay,and HE staining was used to observe the morphology of ovarian tissue of the mice in each group. Results Compared with control group,the levels of estradiol (E2) and testosterone (T) were significantly increased(P<0.05) in 25 and 125 mg/kg ATR groups, and the levels of E2 and T  were  increased with the increasing of ATR dose.Compared with control group,the levels of  luteinizing hormone (LH) and follicle stimulating hormone (FSH)  were significantly decreased (P<0.05 or P<0.01) in 25 and 125 mg/kg groups, especially in  125 mg/kg group (P<0.01).The levels of progesterone (P) and prolactin (PRL) had no significant differences between each group(P>0.05). Compared with control group,the proportions of the development follicles were  reduced in 25 and 125 mg/kg ATR groups, and the proportions of the large atretic follicles were increased,especially in  125 mg/kg ATR group. Conclusion ATR may increase the levels of serum E2 and T  and decrease the  secretion of  LH and FSH in order to interfer  of the ovarian follices.

Key words: atrazine, sex hormone, female mice, follicular development