Abstract:

Abstract:Objective
To study the effects of Yunnan Baiyao and iodoform for rat cleft palate operation,and to find  more suitable drug for the cleft palate operation.Methods 90 SD rats were randomly divided into  control group,drug iodoform group,and Yunnan Baiyao group;there were 30 rats in each group.Through the method of operation,the rat  cleft palate defects models were set up.According to the grouping,the 4 mg Yunnan Baiyao or 4 mg iodoform werer put into the defects,and nothing in  control group.The body temperature of the  rats was detected with anal temperature measurement.Bone density meter was used to detect   the  palate operation area bone density.HE staining and modified Gomori staining were used to observe new bone formation.The contents of bFGF and BMP2 were determined by immunohistochemical method.Results The body temperature of the rats in iodoform group was higher than those in the  other two groups 1 week after operation (P<0.05).The X-ray detection results showed that  the bone density in  Yunnan Baiyao group was higher than those in the other two groups 2 and 3 weeks after operation(P<0.05).The fibroblast  division,proliferation and new bone formation were enhaced in  Yunnan Baiyao group by HE and modified Gomori special staining.The contents of  bFGF and BMP2 in  Yunnan Baiyao group were higher than those in the other two groups 2 and 3 weeks after operation(P<0.05).Conclusion Yunnan Baiyao has no irritation to temperature,and it can not only promote the cleft palate soft tissue healing,but also reduce palatal fistula complications,and it can promote the bony repair of cleft palate.

Key words: rats,sprague-dawley, cleft palate, Yunnan Baiyao, iodoform, bone healing