Journal of Jilin University Medicine Edition ›› 2015, Vol. 41 ›› Issue (03): 573-577.doi: 10.13481/j.1671-587x.20150327

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Regulation effect of 5-HT7 receptor on electrical activity of 5-HT neurons in nucleus raphes dorsalis of rats with Parkinson's disease

WANG Shuang1, GAO Jie2, GUO Yufang1, WANG Xiang1   

  1. 1. Department of Pathophysiology, Institute of Basic Medical Sciences, Xi'an Medical University, Xi'an 710021, China;
    2. Department of Management, Xi'an Medical University, Xi'an 710021, China
  • Received:2014-10-13 Published:2015-08-01

Abstract:

Objective To investigate the different effects of 5-hydroxytryptamine-7(5-HT7) on the electrical activities of 5-HT neurons in nucleus raphes dorsalis(DRN) of the normal and parkinson's disease(PD) rats, and to clarify the properties of 5-HT7 receptor and the changes in the PD rats. Methods 28 male SD rats were randomly divided into sham opration group (n=12) and PD group (n=16).6-OHDA was injected in the subtantia nigra pars compacta(SNc)of the rats in PD group, and the same dose of normal saline was injected in the SNc of the rats in sham opration group.The rats in two groups were injected with different doses of (40-640 μg·kg-1, i.v.) 5-HT7 receptor agonist AS19, and the changes of firing rates in 5-HT neurons of the rats were observed by extracellular electrophysiological recording in DRN.5-HT7 receptor antagonist SB269970 was injected to observe the sensitivities to agonist and the antagonist of the rats in PD group, and compared with sham operation group. Results Systemic administration of the selective 5-HT7 receptor agonist AS19(40-640 μg·kg-1, i.v.)increased the firing rate of 5-HT neurons of the rats in sham operation group, the mean firing rate of 5-HT neurons in the DRN was changed to 2.35±0.16(P<0.001), and completely reversed by the subsequent administration of SB269970(200 μg·kg-1, i.v.), the mean firing rate of 5-HT neurons in the DRN was changed to 0.37±0.03.The same dose of AS19 had no affection in the rats in PD group(P=0.218). Conclusion The regulation effect of 5-HT7 receptor on the 5-HT neurons in DRN of the PD rats is decreased.

Key words: 5-hydroxytryptamine-7 receptor, nucleus raphes dorsalis, 5-hydroxytryptamine neurons, electrophysiology

CLC Number: 

  • R338.2