赖氨大黄酸对D-半乳糖致衰老模型小鼠的肾脏保护作用及其作用机制

doi:10.13481/j.1671-587x.20150402

Abstract

Abstract:

Objective To investigate the protective effect of rhein lysinate (RHL) on the kidney of aging mice induced by D-galactose (D-gal) and to clarfy its mechanism,and to provide the basis for research on prevention of RHL on aging. Methods The models of aging mice were established by intraperitoneally injecting with D-gal.The mice were randomly divided into control,aging model,low and high doses of RHL groups.In addition to the control group,the mice in the other groups were injected subcutaneously with D-gal daily for 8 weeks,while the mice in control group were injected subcutaneously with the same amount of saline daily,and the mice in low and high doses of RHL groups were given 25 and 50 mg·kg^-1 RHL daily by gavage.The contents of malonaldehyde (MDA) and the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in serum and kidney tissue were detected by microplate reader according to the instruction of respective kits.The pathological changes of kidney tissue were observed by hematoxylin and eosin (HE) staining;the expression levels of age-related proteins were detected by Western blotting method. Results Compared with control group,the kidney index of the mice in aging model group was decreased (P<0.05);the activities of SOD and GSH-Px in serum and kidney tissue were decreased (P<0.05),while the content of MDA was increased (P<0.05).Compared with aging model group,the kidney indexes of the mice in 25 and 50 mg·kg^-1RHL groups were increased (P<0.05);the activities of SOD and GSH-Px in serum and kidney tissue of the mice were increased (P<0.05) and the contents of MDA were decreased (P<0.05).Compared with aging model group,the edema of renal tubular epithelial cells in kidney tissue was decreased in a dose-dependent manner in 25 and 50 mg·kg^-1 RHL groups.Compared with aging model group,the expression levels of SIRT1 in kidney tissue of the mice in 25 and 50 mg·kg^-1 RHL groups were increased (P<0.05),while the expression levels of p16 and p21 were decreased (P<0.05). Conclusion RHL can inhibit the oxidative stress and the edema of renal tubular epithelial cells and regulate the expressions of age-related genes to protect the kidney of aging mice.

Key words: rhein lysinate, anti-aging, aging model, D-galactose

CLC Number:

R339.34

WEI Jie, LI Kaiji, ZHAO Yufang, LUO Guangling, WEI Jingbo, YAN Feng, LV Cuiping, ZHEN Yongzhan. Protective effect of rhein lysinate on kidney of aging model mice induced by D-galactose and its mechanism[J].Journal of Jilin University Medicine Edition, 2015, 41(04): 680-684.

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Protective effect of rhein lysinate on kidney of aging model mice induced by D-galactose and its mechanism

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