Abstract: Abstract:Objective To establish conventional PCR and fluorescence quantitative PCR(FQ-PCR) methods for detection of NDM1 gene and to compare the detection results of the two methods.Methods Three pairs of primers that specifically recognized the NDM1 gene were respectively designed,and their reaction systems and parameters were confirmed.Under the optimized conditions,the conventional PCR  and FQ-PCR methods were evaluated for the specificity,sensitivity and detection effects on simulative clinical samples.Results  Conventional PCR and FQ-PCR methods were successfully established.The results of specificity analysis showed that conventional PCR and FQ-PCR  both presented  negative resuts  for seven familiar pathogenic bacteria.The threshold of FQ-PCR was 104 mL-1 and that of conventional PCR was 10⁶ mL^-1.The detection results of s
imulative clinical samples indicated that they had accordant results.Conclusion Both conventional PCR and FQ-PCR  methods possess good specificity,and the sensitivity of FQ-PCR is 100 times higher than conventional PCR.

Key words: new delhi metallo-&beta, -lactamas 1 gene, conventional PCR, fluorescence quantitative-PCR