Abstract:

Objective To explore the inhibitory effect of manumycin on proliferation of human myeloid leukemia cell line K562 and provide the theoretical basis for manumycin in treatment of leukemia.Methods Control group (K562 plus 10% fetal bovine serum containing IMDM medium) and manumycin group (the fatal concentrations were 2，4，8，16 μmol/L）were set up in this experiment and were treated for 24，48，72 h separately.Then the K562 cell growth was observed under microscope and the inhibitory rate was measured by MTT.Compared with control group,after 24 h,the survivin protein expressions in K562 cells treated with 4 and 16 μmol/L manumycin were examined by Western blotting method. Results The observation under microscope showed that the K562 cells in control group showed good growth state,well-distributed dense,fixed-size,clear outline and strong refraction.Compared with control group,the cells in 8 μmol/L manumycin group showed smaller size,thin dense,unfixed-size,unclear outline and weakened refraction;the cells in 16 μmol/L manumycin group showed less cell dense,anisocytosis,unclear cell outline and cell fragments.The MTT results showed that compared with control group,the inhibitory rate of K562 cells was raised with the drug concentration increasing and exposed time prolongation （P<0.05 or P<0.01），exhibiting a time-dose-effect relationship.The Western blotting results showed that compared with control group,after 24 h, the survivin protein expression in 16 μmol/L manumycin group was decreased.Conclusion Manumycin can stimulate apoptosis and inhibit tumor cell growth by down-regulating survivin protein expression and cure leukemia.

Key words:  manumycin;leukemia;survivin;K562 cell line