Abstract:

Objective: To evaluate the role of the multiple tumor markers protein chip (C12 chip) in healthy screening of old people,and to quantitatively analyze the changes and clinical significance of the detection values and positive rates of tumor markers of the subjects with different ages and genders. Methods: A total of 9 654 cases were selected. According to the age,the subjects were divided into control group(aged 40 to 59),old people group(aged 60 to 79)and senile people group(aged 80 and above).The protein chip system for multiple tumor marker detection(Chip C-12) was used to measure the levels of 12 tumor markers including alpha fetoprotein(AFP),carcinoembryonic antigen(CEA),carbohydrate antigen 15-3(CA15-3),carbohydrate antigen 19-9(CA19-9),carbohydrate antigen 125(CA125),carbohydrate antigen 242(CA242),ferritin,human chorionic gonadotropin beta(β-HCG),human growth hormone(HGH),neuron specific enolase (NSE),prostate special antigen(PSA) and free PSA(f-PSA).The positive result was confirmed when the levels of one or more tumor markers were higher than the reference value.The detection values and the positive rates of the subjects in three groups were analyzed. Results: 1Among 9 654 cases,the serum levels of tumor biomarkers were found to be positive in 665 cases,and the positive rate was 6.89%;among them 32 cases were diagnosed as early or intermediate cancer through endoscope,pathological and imageological diagnosis,and the detection rate was 0.33%.2 The positive rates were different in different genders. In male,the top five order was f-PSA,PSA,CEA,CA19-9, and NSE;while in female,the top five order was CA19-9,CA125,CEA,NSE and CA242.3positive rates of CA19-9,CA242,CEA,NSE,PSA and f-PSA were increased with the increasing of age(P<0.01).4The values of AFP,CA125,CA19-9,CA242,CEA,HGH,NSE,and PSA were increased with the increasing of age; the above indexes of the subjects in senile people group were higher than those in old people group and control group(P<0.01),and the above indexes of the subjects in old group were higher than those in control group (P<0.01). Conclusion: The multiple tumor markers (CA19-9,CEA,NSE,PSA,and f-PSA) of C12 chip are useful in the healthy screening of the old people, especial in the senile people to discover tumor at an early stage.

Key words: protein chip, multiple tumor markers, senile people, healthy screening