DNMT3b基因多态性及其在胃癌组织中的表达水平与胃癌的关联性分析

doi:10.13481/j.1671-587x.20150232

Abstract

Abstract:

Objective To investigate the associations between single nucleotide polymorphisms(SNPs)of DNA methyltransferase 3b(DNMT3b) gene and the risk of gastric cancer in the Han population from North China,and to clarify whether DNMT3b gene is the susceptible gene of gastric cancer.Methods 447 patients with gastric cancer were selected as case group and 961 healthy controls were used as control group.The distribution of genotypic frequencies of five SNPs(rs6119954,rs1569686,rs4911107,rs4911259,and rs8118663)in two groups were detected by TaqMan assay.The expression levels of DNMT3b protein in 104 cases of cancer tissue from the patients with gastric cancer and 85 cases of tumor-adjacent tissue from the patients with gastric cancer were analyzed by immunohistochemistry method.Results The distributions of genotypic frequencies of five SNPs in case group and control group were similar.No significant association was found between the five SNPs and the risk of gastric cancer(P>0.05).Compared with the tumor-adjacent tissue,the expression level of DNMT3b protein in the gastric cancer tissue was increased(P<0.05).There were no siginificant differences between the genotypes of the five SNPs and the expression levels of DNMT3b protein(P>0.05).Conclusion There is no association between the polymorphisms of DNMT3b and the occurrence of gastric cancer in Han population from North China.DNMT3b gene may not be the susceptible gene of gastric cancer.

Key words: DNA methyltransferase 3b, stomach neoplasms, sigle nucleotide polymorphisms

CLC Number:

R735.2

WANG Chuan, JIA Zhifang, CAO Donghui, WU Xing, CAO Xueyuan, JIANG Jing. Associations between polymorphisms of DNMT3b gene and itsexpression level in gastric cancer tissue and gastric cancer[J].Journal of Jilin University Medicine Edition, 2015, 41(02): 369-373.

References

[1] Ferlay J,Shin HR,Bray F,et al. Estimates of worldwide burden of cancer in 2008:GLOBOCAN 2008 [J].Int J Cancer,2010,127(12):2893-2917.

[2] Saeki N,Ono H,Sakamoto H,et al.Genetic factors related to gastric cancer susceptibility identified using a genome-wide association study [J].Cancer Sci,2013,104(1):1-8.

[3] Jeltsch A.Molecular enzymology of mammalian DNA methyltransferases [J].Curr Top Microbiol Immunol,2006,301:203-225.

[4] 张丽丽,吴建新.DNA甲基化——肿瘤产生的一种表观遗传学机制 [J].遗传,2006, 28(7):880-885.

[5] 殷科,盛贤能,郭宇,等.DNMT1、DNMT3b在胃癌中的表达及其临床意义 [J].现代实用医学,2013,25(12):1386-1388.

[6] Daraei A,Salehi R,Mohamadhashem F.DNA-methyltransferase 3B 39179 G > T polymorphism and risk of sporadic colorectal cancer in a subset of Iranian population [J].J Res Med Sci,2011,16(6):807-813.

[7] Lee SJ,Jeon HS,Jang JS,et al.DNMT3B polymorphisms and risk of primary lung cancer [J].Carcinogenesis,2005,26(2):403-409.

[8] Samudio-Ruiz SL,Hudson LG.Increased DNA methyltransferase activity and DNA methylation following epidermal growth factor stimulation in ovarian cancer cells [J].Epigenetics,2012,7(3):216-224.

[9] 鲍倩,何帮顺,陈丽萍,等.DNA甲基转移酶3B基因启动子单核苷酸多态性与结直肠癌的相关性 [J].中华预防医学杂志,2012,46(1):53-57.

[10] Wang YM,Wang R,Wen DG,et al.Single nucleotide polymorphism in DNA methyltransferase 3B promoter and its association with gastric cardiac adenocarcinoma in North China [J].World J Gastroenterol,2005,11(23):3623-3627.

[11] Hu JB,Fan H,Liu DS,et al.DNMT3B promoter polymorphism and risk of gastric cancer [J].Dig Dis Sci,2010,55(4):1011-1016.

[12] Shen HB,Wang L,Spitz MR,et al.A novel polymorphism in human cytosine DNA-methyltransferase-3B promoter is associated with an increased risk of lung cancer [J].Cancer Res,2002,62(17):4992-4995.

[13] Montgomery KG,Liu MC,Eccles DM,et al.The DNMT3B C→T promoter polymorphism and risk of breast cancer in a British population:a case-control study [J].Breast Cancer Res, 2004,6(4):390-394.

[14] Yang XX,He XQ,Li FX,et al.Risk-association of DNA methyltransferases polymorphisms with gastric cancer in the Southern Chinese population [J].Int J Mol Sci,2012,13(7):8364-8378.

[15] Aung PP,Matsumura S,Kuraoka K,et al.No evidence of correlation between the single nucleotide polymorphism of DNMT3B promoter and gastric cancer risk in a Japanese population [J].Oncol Rep,2005,14(5):1151-1154.

[16] Su XW,Lv CY,Qiao FC,et al.Expression pattern and clinical significance of DNA methyltransferase 3B variants in gastric carcinoma [J].Oncol Rep,2010,23(3):819-826.

[17] 周宁,李象霖,张月萍,等.甘肃地区人群雌激素受体α-29位基因多态性与HBV感染转归的关系[J].临床肝胆病杂志,2014,30(2):128-131.

Associations between polymorphisms of DNMT3b gene and itsexpression level in gastric cancer tissue and gastric cancer

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 0

[1]	ZHAO Dan, CAO Donghui, JIN Meishan, WU Menghui, WANG Yueqi, YANG Na, ZHOU Tianyu, ZHANG Houjun, JIANG Jing, CAO Xueyuan. Inhibitory effect of 18β-glycyrrhetinic acid on inflammation-related gastric cancer and its mechanism [J]. Journal of Jilin University(Medicine Edition), 2018, 44(06): 1150-1155.
[2]	YANG Dongye, JIAO Yang, TAN Bibo, DU Zhijian, HU Xiaojie, LIU Fei. Expression of Gli1 in gastric carcinoma tissue and its effect on biological characteristics of gastric carcinoma cells [J]. Journal of Jilin University Medicine Edition, 2018, 44(01): 63-67.
[3]	LIU Gang, ZHAO Minxue, WANG Desheng. Application of detection of serum pepsinogen, gastrin-17 and Helicobacter pylori antibody in gastric cancer screening [J]. Journal of Jilin University Medicine Edition, 2017, 43(06): 1182-1185.
[4]	KONG Guimei, WANG Jijun, TAO Wenhua, BU Ping, QIAN Feng. Expression of PRMT5 in human gastric cancer tissue and its significance [J]. Journal of Jilin University Medicine Edition, 2016, 42(04): 753-756.
[5]	YOU Lili, CAO Donghui, JIANG Jing, LIU Ruming, MA Lin, WANG Shidong, SUO Yueer, HOU Zhen, CAO Xueyuan. Inhibitory effect of 18β-glycyrrhetinic acid on gastric tumor in K19-C2mE transgenic mice and its mechanisms [J]. Journal of Jilin University Medicine Edition, 2016, 42(02): 221-225.
[6]	JI Guofeng, TAO Youmao, LI Zhuo, MA Chong, XIAO Ling. Analysis on risk factors of infections in patients with gastric cancerafter laparoscope-assisted distal gastrectomy [J]. Journal of Jilin University Medicine Edition, 2015, 41(05): 1076-1079.
[7]	WU Xing, JIA Zhifang, YOU Lili, CAO Donghui, WANG Chuan, CAO Xueyuan, JIANG Jing. Influence of single nucleotide polymorphisms of DNMT1 gene in survival of patients with gastric cancer after operation [J]. Journal of Jilin University Medicine Edition, 2015, 41(04): 802-807.
[8]	LI Chunmei, ZHANG Lin, HOU Yanhong, LI Nan. Influence of potential tumor suppressor gene TXNIP in biological characteristics of gastric cancer MKN-45 cells [J]. Journal of Jilin University Medicine Edition, 2015, 41(03): 588-592.
[9]	CAI Jian-ying，YANG Xiao-dong，ZHOU Ming-chuan. Influence of restoration of miR-21 expression level in CD44 mRNAand 　protein expressions in human gastric cancer Kato Ⅲ cells [J]. Journal of Jilin University Medicine Edition, 2013, 39(4): 751-754.
[10]	CUI Dian-long\|XIE Bai-yi\|HU Meng\|XIANG Xin\|BAO Chuan-en,CHEN Yu-qiang. Inhibitory effect of tetraiodothyroacetic acid on proliferation of drug- resistant human gastric cancer cell line in vivo and in vitro [J]. J4, 2012, 38(2): 281-285.
[11]	PENG Yun-xiang,MAO Jing-tao,WEI Jun,HOU Zhi-fu. Evidence-based analysis of CK19 and EpCAM expressions in gastric cancer tissues [J]. J4, 2011, 37(2): 296-299.
[12]	. Anti-tumor effect of extract from \|Bark of Juglans \|Mandshurica Maxim 　on gastric cancer mice in vivo [J]. J4, 2011, 37(2): 260-263.
[13]	SONG Ming-Xu, LI Li-Hua, DING Su-Ning, LIU Zhi-Hui, ZHOU Xi-Ke. Expressions of platelet-derived growth \|factors in primary adenocarcinoma of stomach tissues \|and significance [J]. J4, 2011, 37(1): 97-101.
[14]	FENG Yun-xia,HOU Rui-sheng,ZHANG Xiao-hui,WANG Kai-juan. Expression of Prohibitin in gastric cancer tissue and its clinical significance [J]. J4, 2008, 34(4): 649-653.
[15]	SUN Yan-xia,YANG Shao-juan,GAO Shen,SHI Zhang-zhen,LU Zhen-xia. Inhibitory effect of silencing survivin gene with siRNA on growth of human gastric cancer SGC-7901 cells [J]. J4, 2008, 34(3): 477-481.