Abstract:

Objective To extract the  Ginseng polysaccharide (GPS),polysaccharides of Tricholoma matsutake(PTM) and polysaccharide of Lentinus edodes
 (PLE) from gingeng,tricholoma matsutake and lentinus edodes respectively,and to analyze and identify their structures,and to prepare their complex,and to study the indirect antitumor activity in vitro  of polysaccharide complex.Methods The polysaccharides were extracted with hot water and precipitated by etha
nol.The carbohydrate levels were determined by the method of phenol-sulfuric acid.The  m-hydroxyphenyl method was used to determine the levels of  uronic acid,and the national standard method was used to determine the levels of starch.Infrared spectroscope and chemical methods were performed to analyze their structures. Orthogonal experiment was used to study mixing methods.Cytotoxic T lymphocyte experiment and LDH release assay were performed to detect the influence of polysaccharide complex of  GPS,PTM,and PLE in the CTL killing activity,and its indirect killing effect on the P815 cells.
Results The extraction rates of  GPS,PTM,and PLE were 8.85%,9.40%,and 10.50%; the levels of total polysaccharides were 62.96%,59.13%,and 33.86%;
the levels of uronic acid were 16.44%,9.37%,and 16.44%;the starch levels were 7.26%,2.80%， and 3.77%,respectively.The identification results showed that the polysaccharides were obstrained.When the quality ratio of the three kinds of polysaccharides was 1∶1∶1 and the concentration was 600 mg/L,the CTL cytotoxicity was the highest.Conclusion The polysaccharide complex is obtained, identified and characterized. Polysaccharide complex can enhance the cytotoxicity of CTL and has the indirectly inhibitory effect on the proliferation of P815 cells.

Key words: Ginseng polysaccharide;polysaccharides of Tricholoma matsutake;polysaccharide of Lentinus edodes;polysaccharide complex; , antitumor activity