Mu2蛋白亚细胞结构定位及其相互作用蛋白的筛选

doi:10.13481/j.1671-587x.20160603

Abstract

Abstract:

Objective: To express the FLAG-Mu2 protein in drosophila S2 cells,and to investigate its subcellular localization and to purify and identify the FLAG-Mu2 protein complex,and to provide basis for the study of Mu2 protein.Methods: The vector was transfected into drosophila S2 cells. The subcellular localization of FLAG-Mu2 protein was tested by confocal laser scanning microscope. The expression levels of FLAG-Mu2 protein in nuclear extractions were examined using nuclear and cytoplasmic protein extraction method. The FLAG-Mu2 complex was preciptated by precipitation technology and purified by chromatographic steps and the components were analyzed by mass spectrometry.Results: The confocal laser scanning microscope results showed that the FLAG-Mu2 protein mainly expressed in nucleus.The nuclear and cytoplasmic protein extraction results showed that the fusion protein,with a relative molecular weight of 140 000,mainly existed in nuclear extraction. The FLAG-Mu2 complex was purified and analyzed by mass spectrometry,and its results showed that there were 5 components,including pif1A,CG31782,mip120,CG31690,and G protein-sa60A,may be included in FLAG-Mu2 complex.Conclusion: The FLAG-Mu2 protein mainly localizes in the nucleus of drosophila S2 cells; a total of 5 kinds of proteins are found to be potential components of FLAG-Mu2 protein complex.

Key words: Mu2 protein, fusion protein, subcellular localization, screening of interacting proteins

CLC Number:

Q291

HUO Yunlong, WANG Chunyu, ZHAO Yue. Subcellular localization of Mu2 protein and screening of its interacting proteins[J].Journal of Jilin University Medicine Edition, 2016, 42(06): 1054-1058.

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Subcellular localization of Mu2 protein and screening of its interacting proteins

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