粗毛纤孔菌对寒凝血瘀证大鼠血液流变学的影响及谱效关系分析

doi:10.13481/j.1671-587x.20180106

Abstract

Abstract: Objective: To explore the effects of the activating circulation to remove blood stasis through observingthe influence of Inonotus hispidus on hemorheology in the rat models with blood stasis due to cold syndrome.. Methods: The rats were randomly divided into normal control group, blood stasis model group, positive control group, low and high doses ofInonotus hispidus(S1-S6) sample groups; there were 15 groups,and 12 rats were in each group. Except for normal control group, the rats in other groups were injected adrenaline hydrochloride and immersing in ice water to build the blood stasis due to cold syndrome rat models.The indexes of hemorheoloy of the rats were detected,and the HPLC spectrum-effect relationship was analyzed. Results: The yellow fruiting bodies(S3-S6) of Inonotus hispidus which were parasitic on the different trees significantly improved the hemorheological indexes in the rat models.Compared with model group, the blood viscosity and whole blood viscosity of the rats in high dose of yellow fruiting body of Inonotus hispidus group were decreased(P<0.05).The black fruiting body of Inonotus hispidus showed the similar effect to the yellow fruiting body, but the hemorheological indexes of the rats in black fruiting body of Inonotus hispidus group had no significant changes compared with model group(P>0.05). The results of the spectrum-effect showed that the corresponding components of 11 chromatographic peaks were closely correlated with the changes of plasma viscosity, and the correlation coefficient > 0.8.Through identification,four compounds of them were named phellibaumin A,phelligridin C(cis),phelligridin C(trans),phelligridin D and 3'4'-dihydroxy-5-[(11-hydroxyphenyl)-6,7-vinyl]-3,5-dioxa-fluoren-5-one. Conclusion: The yellow fruiting bodies of Inonotus hispidus which are parasitic on the different trees can decrease the blood viscosity and whole blood viscosity and have the effects of activating the circulation to remove blood stasis.

Key words: spectrum-effect relationship, blood stasis due to cold syndrome, hemorheology, Inonotus hispidus

CLC Number:

R285.5

LI Qingjie, BAO Haiying, BAU Tolgor, LI Yu. Influence of Inonotus hispidus in hemorheology in rat model with blood stasis due to cold syndrome and analysis on spectral efficiency[J].Journal of Jilin University Medicine Edition, 2018, 44(01): 30-35.

References

[1] 郑立军,王清,季俊,等.药用真菌-桑黄的研究进展[J].现代中药研究与实践,2005,19(3):61-64.
[2] 昝立峰,包海鹰,图力古尔.粗毛纤孔菌子实体化学成分[J].菌物学报,2013,32(1):150-156.
[3] 昝立峰,包海鹰.粗毛纤孔菌的研究进展[J].食用菌学报,2011,18(1):78-82.
[4] 史帧婷,包海鹰.桑黄类真菌有效成分及功效研究进展[J].中国实验方剂学杂志,2016, 22(22):197-202.
[5] 王婷,包海鹰,图力古尔.粗毛纤孔菌固体发酵菌粉对H22荷瘤小鼠抗肿瘤作用研究[J]. 中国实验方剂学杂志,2016,39(2):389-394.
[6] 昝立峰,包海鹰,李丹花."桑黄"类真菌中多酚物质及其生物活性研究进展[J].天然产物研究与开发,2016,28(1):147-155.
[7] 张媛,包海鹰.四种多孔菌子实体粗多糖抗肿瘤活性的比较研究[J].菌物学报,2014,33(1):114-120.
[8] 昝立峰,梁瑞娟,包海鹰.粗毛纤孔菌提取物的抗氧化和抑菌活性研究[J].北方园艺,2015(5):151-155.
[9] 黄孝闻,俞忠明,董宇.桑黄在古代中医典籍中的应用记载及其现代药用研究进展[J].中华中医药学刊,2014,32(9):2249-2251.
[10] 张玉.复方人参挥发油气雾剂抗心肌缺血机制及对血液流变学影响的研究[D].长春:长春中医药大学,2016.
[11] 孟莉,焦富英,向绍杰.脑栓通颗粒活血化瘀作用的实验研究[J].中成药,2011,33(3):407-410.
[12] 赵春芳,李庆杰,王莲萍.返魂草的高效液相色谱法指纹图谱研究及成分分析[J].分析化学,2013,44(1):133-136.
[13] 贾红岩,王亚涛,张芝华,等.高效液相色谱法测定不同产地及品种灵芝三萜类成分的含量[J].微生物学通报,2017,44(1):238-244.
[14] 苏春萌,唐嘉熙,罗阳.柴胡注射液解热作用的谱-效关系研究[J].中国药房,2016,27(10):1340-1343.
[15] 吕邵娃,董书羽,郭玉岩,等.数据分析技术在中药谱效关系中的应用进展[J].中国实验方剂学杂志,2015,21(15):226-230.
[16] 王婷,包海鹰,图力古尔,等.寄生于蒙古黄榆上的粗毛纤孔菌生物学特性及驯化栽培[J].菌物学报,2016,35(6):695-704.
[17] 戴玉成,图力古尔.中国东北野生食药用真菌图志[M].北京:科学出版社,2007.
[18] 李雨沁.粗毛黄褐孔菌人工驯化栽培及其降血糖、抗氧化活性研究[D].阿拉尔:塔里木大学,2010.
[19] Awadh N, Mothana RA, Lesnau A, et al. Antiviral activity of Inonotus hispidus[J]. Fitoterapia, 2003, 74(5):483-485.
[20] 王占斌,孙常雁,李德海,等.粗毛纤孔菌子实体多糖的提取及免疫功能研究[J].食品工业科技,2011,32(12):384-386.
[21] 杨璐,李庆华,许玲,等.粪菌移植对阿尔茨海默病小鼠学习记忆能力的影响[J].郑州大学学报:医学版,2017,52(6):702-706.
[22] 向青青,芦起,余加林,等.密度感应系统基因lasR/rhlR对大鼠铜绿假单胞菌生物膜感染气管插管模型肺组织Foxp3、TGF-β和IL-10表达的影响[J].解放军医学杂志,2016,41(2):103-107.

Influence of Inonotus hispidus in hemorheology in rat model with blood stasis due to cold syndrome and analysis on spectral efficiency

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