Abstract:

Objective To explore the apoptosis inducing effect of vincristine on human osteosarcoma MG63 cells and clarify the molecular mechanisms.Methods The  apoptosis and intracellular reactive oxygen species(ROS) level of MG63 cells were determined by flow cytometry,after incubation with Annexin V-PI and DCFH-DA which were explored on different concerntrations of vincristine (0,5,10,20,50,100 μg/L).Real-time quantitative RT-PCR was used to analyze gene expression.Results 10 μg/L vincristine could significantly increase the apoptotic rate of MG63 cells to 17.6%(P<0.05),the apoptotic rate was increased with the increasing of concentration of vincristine in a dose-dependent manner;5 μg/L vincristine increased the intracellular ROS level and decreased the expression of CAT gene (P<0.05).Conclusion Vincristine can promote the apoptosis of MG63 cells with low concentration,its mechanism is to down-regulate the expresion of CAT gene and induce the increase of ROS to promote apoptosis

Key words: MG63;Vincristine;Apoptosis;reactive oxygen species;CAT