BRD4基因对TGF-β1诱导的心肌细胞凋亡和p38MAPK信号通路的影响

doi:10.13481/j.1671-587x.20190419

Abstract

Abstract: Objective:To investigate the effect of bromodomain-containing protein 4(BRD4) gene on the apoptosis of cardiomyocytes induced by transforming growth factor-β1 (TGF-β1), and to clarify its possible mechanism. Methods:The H9c2 cells were randomly divided into blank control group,si-CN group (transfected with negative control siRNA),and si-BRD4 group (transfected with specific siRNA).The expression levels of BRD4 protein in the cells in various groups were detected by Western blotting method.The H9c2 cells were randomly divided into control group, TGF-β1 group (transfected with 20μg·L^-1TGF-β1 for 24 h), si-NC+TGF-β1 group (treated with TGF-β1 for 24 h after transfected with negative control siRNA) and si-BRD4+TGF-β1 group (treated with TGF-β1 for 24 h after transfected with BRD4 specific siRNA).The cell proliferation activity was measured by MTT assay, and the apoptotic rate of cells was detected by Annexin Ⅴ-FITC/PI double staining. Western blotting method was used to detect the expressions of BRD4, Bcl-2, Bax, Cleaved caspase 3, P38, and p-P38 proteins. Results:Compared with blank control group, the expression level of BRD4 protein in the H9c2 cells in si-BRD4 group was significantly decreased (P<0.01).Compared with control group, the cell proferation activity and the Bcl-2 protein expression level in TGF-β1 group were significantly decreased(P<0.01); the apoptotic rate, and the expression levels of Bax, Cleaved caspase3, and p-p38 proteins were significantly increased (P<0.01).Compared with TGF-β1 group, the cell prdiferation activity and the expression level of Bcl-2 protein in si-BRD4+TGF-β1 group were significantly increased (P<0.05), while the apoptotic rate and the expression levels of Bax, Cleaved caspase3, and p-p38 proteins were significantly reduced(P<0.05). Conclusion:Inhibition of BRD4 gene expression can attenuate the apoptosis of cardiomyocytes induced by TGF-β1, which may be related to the inhibition of p38MAPK signaling pathway.

Key words: cardiomyocyte, bromo domain protein 4, transforming growth factor 1, p38MAPK signaling pathway

CLC Number:

YANG Dongwei, GUI Yunong. Effects of BRD4 gene on apoptosis and p38MAPK signaling pathway of cardiomyocytes induced by TGF-β1[J].Journal of Jilin University(Medicine Edition), 2019, 45(04): 855-860.

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Effects of BRD4 gene on apoptosis and p38MAPK signaling pathway of cardiomyocytes induced by TGF-β1

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