Abstract:

Abstract:Objective To study the intervention of fluticasone combined with ventolin and montelukast sodium on rat asthmatic airway remodeling and clarify the mechanisms of asthma and airway remodeling. Methods Forty Wistar rats were randomly divided into negative control group, positive control group(model), drug 1 group(inhaled with fluticasone and ventolin) and drug 2 group(administered with montelukast sodium),10 rats in each group. OVA was used to set up rat asthma airway remodeling.The number of inflammatory cells in bronchoalveolar lavage fluid(BALF) was observed, the serum IL-12 levels were detected in various groups, and the changes of ciliated epithelial cells, fibroblasts, and smooth muscle cells were also observed. Results Compared with negative control group, the number of inflammatory cells in rat BALF in positive control group was significantly increased(P<0.05); compared with positive  control group, the number of inflammatory cells in rat BALF in drug 1 group and drug 2 group was significantly decreased (P<0.05); but there was no significant difference between drug 1 group and drug 2 group(P>0.05). Compared with negative control group, the serum IL-12 level of rats in positive control group was decreased(P<0.05); compared with positive control group, the serum IL-12 levels of rats in drug 1 group and drug 2 group were significantly increased (P<0.05 or P<0.01);and there was significant difference between drug 1 group and drug 2 group (P<0.01). Compared with drug 2 group, the airway cilia arranged regularly, the elastic fibers were basically normal, the karyon and cytoplasm had no changes, the smooth muscle mildly thickened, the lymphocytes moderately infiltrated indrug 1 group. Conclusion Fluticasone combined with ventolin can inhibit the infiltration of inflammatory cells, alleviate the airway inflammation, weaken asthma  airway remodeling, and decrease the high airway reactivity to protect the morphology and function of airway.

Key words: asthma; , airway remodeling; , rats,Wistar