Abstract:

To study the effects of deoxycytidine kinase (DCK) on drug sensitivity and radiosensitivity of human cervical carcinoma cell line HeLa and to provide theoretical basis for gene therapy of cervical carcinoma. Methods FuGENE 6 liposome was used to transfect HeLa and establish RNAi models,the cells were divided into control,empty-vector and DCK-siRNA group,the expressions of DCK mRNA and protein before and after transfection were detected by quantitative real-time PCR and Western blotting,the abilities of proliferation were measured by cell counting,the drug sensitivity to cytarabine（AraC） was determined by MTT assay,the clonogenic formation test was used to assess the radiosensitivity.Results Compared with control group and empty-vector group, the    expressions of DCK mRNA and protein in siRNA group were significantly decreased (P<0.05).There was no significant difference of growth curve among three groups.The drug sensitivity to AraC in siRNA group was decreased,while the radiosensitivity was increased (P<0.05).Conclusion Inhibition of DCK could reduce the drug sensitivity and increase the radiosensitivity in human cervical carcinoma cells.

Key words: deoxycytidine kinase;HeLa cells;drug sensitivity;radiosensitivity